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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
- 亚型:
IgG
- 形态:
liquid
- 保存条件:
负20摄氏度
- 克隆性:
Polyclonal antibody
- 标记物:
Non-conjugated
- 适应物种:
Human,Mouse ,Rat
- 保质期:
6个月
- 抗原来源:
Rabbit
- 目录编号:
Q9BZJ3
- 级别:
纯化级别
- 库存:
50
- 供应商:
LSM bio
- 宿主:
E. coli - derived recombinant protein
- 应用范围:
ELISA,WB,IHC,IF,FC
- 浓度:
≥95% as determined by SDS-PAGE
- 靶点:
ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
- 抗体英文名:
anti-UBC9-Specific antibody,UBC9-Specific antibody
- 抗体名:
anti-UBC9-Specific 抗体,UBC9-Specific 抗体
- 规格:
100μl
UBC9-Specific抗体| UBC9-Specific antibody
货号 PAab09159
蛋白别名 C358B7.1 antibody, E2 antibody,UBE2I antibody, P18 antibody, SUMO conjugating enzyme UBC9 antibody, SUMO protein ligase antibody, UBC9 antibody, UBCE9 antibody, UBE2I antibody, Ubiquitin carrier protein 9 antibody, Ubiquitin carrier protein I antibody, Ubiquitin protein ligase I
蛋白介绍
UBC9 is also named as UBE2I, UBCE9 and belongs to the ubiquitin-conjugating enzyme family. It is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 molecule to the target protein. This protein is present at a high level in spleen and lung. Moderate level of UBE2I is detected in kidney and liver. Low amount of UBE2I is observed in brain, whereas the 18 kDa band of UBE2I is barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the UBE2I antibodies recognizes a 38 kDa protein band,but this band is not visible in extracts of other rat tissues(PMID:14739995).
产品描述
anti-UBC9-Specific antibody is a Rabbit Polyclonal antibody againstUBC9-Specific..
建议稀释比例
WB : 1:500-1:5000;IHC:1:20-1:200;IF:1:10-1:100
IHC
Immunohistochemistry of paraffin-embedded human lung cancer using PAab09159(UBE2I-Specific antibody) at dilution of 1:50
Western blot
HEK-293 cells were subjected to SDS PAGE followed by western blot with PAab09159(UBE2I-Specific antibody) at dilution of 1:400(本抗体仅供体外科研用途,不可用于临床诊断!) "">
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文献和实验Enhanced Detection of In Vivo SUMO Conjugation by Ubc9 Fusion-Dependent Sumoylation (UFDS)
the in vivo sumoylation of a substrate protein at its specific sumoylation site. UFDS utilizes an expression plasmid for the protein of interest fused to the SUMO-conjugating enzyme Ubc9. When expressed in HEK293, COS-7, HeLa, or CHO cells, the fused target
Screening of Antigen-Specific Antibody-Secreting Cells
Screening of antigen-specific antibody-producing cells is a key step for obtaining antigen-specific monoclonal antibodies. In murine system, hybridoma between B-lymphocytes and myeloma cells is used to screen and produce antigen-specific
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