- ￥2260
- LSM Bio
- 进口
- PAab09155
- 2025年07月17日
- ELISA,WB,IHC
- E. coli - derived recombinant protein
- Human,Mouse ,Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
- 亚型：
IgG
- 形态：
liquid
- 保存条件：
负20摄氏度
- 克隆性：
Polyclonal antibody
- 标记物：
Non-conjugated
- 适应物种：
Human,Mouse ,Rat
- 保质期：
6个月
- 抗原来源：
Rabbit
- 目录编号：
Q9BZJ3
- 级别：
纯化级别
- 库存：
50
- 供应商：
LSM bio
- 宿主：
E. coli - derived recombinant protein
- 应用范围：
ELISA,WB,IHC
- 浓度：
≥95% as determined by SDS-PAGE
- 靶点：
ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
- 抗体英文名：
anti-UBC9 antibody,UBC9 antibody
- 抗体名：
anti-UBC9 抗体,UBC9 抗体
- 规格：
100μl
UBC9抗体| UBC9 antibody
货号 PAab09155
蛋白别名
蛋白介绍
产品描述
anti-UBC9 antibody is a Rabbit Polyclonal antibody againstUBC9..
建议稀释比例
IHC
Immunohistochemistry of paraffin-embedded human lung cancer using PAab09155(UBC9 antibody) at dilution of 1:50
Western blot
mouse spleen tissue were subjected to SDS PAGE followed by western blot with PAab09155(UBC9 antibody) at dilution of 1:50(本抗体仅供体外科研用途，不可用于临床诊断!) "">
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文献和实验Enhanced Detection of In Vivo SUMO Conjugation by Ubc9 Fusion-Dependent Sumoylation (UFDS)
The bottleneck in studying protein sumoylation—the conjugation of the small ubiquitin-like modifier (SUMO)—is the detection of the low level of in vivo sumoylated proteins. The Ubc9 fusion-directed sumoylation (UFDS) system strongly enhances
Purification of SUMO Conjugating Enzymes and Kinetic Analysis of Substrate Conjugation
SUMO conjugation to protein substrates requires the concerted action of a dedicated E2 ubiquitin conjugation enzyme (Ubc9) and associated E3 ligases. Although Ubc9 can directly recognize and modify substrate lysine residues that occur
Performing In Vitro Sumoylation Reactions Using Recombinant Enzymes
to perform in vitro sumoylation reactions, namely the E1 activating enzyme Aos1/Uba2 (SAE1/SAE2), the E2 conjugating enzyme Ubc9, SUMO-1 (identical protocols can be used for SUMO-2/3), and the catalytic domain of the E3 ligase RanBP2/Nup358. Two alternative
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