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- 文献和实验
- 技术资料
- 免疫原:
Purified recombinant human AKT protein fragments expressed in E.coli.
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Monoclonal
- 适应物种:
Hu Ms Rt
- 保质期:
12 months
- 抗原来源:
Mouse
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Mouse
- 应用范围:
WB, IF
- 靶点:
Uniprot:P31749
- 抗体英文名:
total AKT Conjugated Monoclonal Antibody
- 规格:
50ul/100ul
配方:0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
应用详情:WB: 1:50-1:200
IF:1:50-1:200
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文献和实验Western Blotting with Monoclonal Ant
the blocking buffer from the blot, add the antibody solution, and incubate with agitation for 30 minutes at 37˚C, one hour at room temperature, or overnight at 4˚C. Enzyme conjugated secondary antibody Note: The inclusion of sodium azide is to be avoided
Western Blotting with Monoclonal Antibodies
the antibody in the corresponding blocking buffer. Decant the blocking buffer from the blot, add the antibody solution, and incubate with agitation for 30 minutes at 37˚C, one hour at room temperature, or overnight at 4˚C. Enzyme conjugated
to about 60°C, then stir until dissolved (solution will be cloudy). Bring to room temperature before using. Primary antibody: For his tagged proteins - Anti-His monoclonal antibody - BD Bioscience #631212
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