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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Synthesized peptide derived from human p65 around the mono-methylation site of K314/K315.
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Polyclonal
- 适应物种:
Hu Ms Rt
- 保质期:
12 months
- 抗原来源:
Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB, IF
- 靶点:
Uniprot:Q04206
- 抗体英文名:
NFκB-p65 (Mono-Methyl-Lys314/Lys315) Polyclonal Conjugated Antibody
- 规格:
50ul/100ul
计算分子量:60
配方:0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
应用详情:WB: 1:50-1:200
IF:1:50-1:200
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文献和实验antibody at 5 µg/ml for 45 min at 4 °C. Mouse anti-human NFB p65 monoclonal antibody sc-8008 (Santa Cruz, CA, USA) recognizes epitopes mapping the C amino-acid terminus of human origin NFB p65. After two washes in PBS–FCS, nuclei were incubated for 45 min
ELISPOT (Enzyme-linked ImmunoSPOT)
Capture Antibody: Pre-titrated, Functional Grade (low endotoxin) purified antibody Detection Antibody: Pre-titrated, biotin-conjugated antibody ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and 0.22 uM filter)
Synthesis and Probing of Membrane-bound Peptide Arrays
in the next section. Stage 6: Protein-binding Assay This basic procedure has been optimized for use with AP-conjugated detection antibody and a color signal development. As mentioned
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