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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
peptide
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Polyclonal
- 适应物种:
Hu,Ms,Rt
- 保质期:
12 months
- 抗原来源:
Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB, IF
- 靶点:
Uniprot:P62736
- 抗体英文名:
Alpha smooth muscle Actin Conjugated Antibody
- 规格:
50ul/100ul
AAT6 antibody
ACTA_HUMAN antibody
ACTA2 antibody
Actin alpha 2 smooth muscle aorta antibody
Actin aortic smooth muscle antibody
Actin, aortic smooth muscle antibody
ACTSA antibody
ACTVS antibody
Alpha 2 actin antibody
Alpha actin 2 antibody
Alpha cardiac actin antibody
Alpha-actin-2 antibody
Cell growth inhibiting gene 46 protein antibody
Cell growth-inhibiting gene 46 protein antibody
GIG46 antibody
Growth inhibiting gene 46 antibody
MYMY5 antibody
计算分子量:42 kDa
配方:0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
应用详情:WB: 1:50-1:200
IF:1:50-1:200
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文献和实验by reaction with alkaline phosphatase-conjugated goat anti-mouse secondary antibody. Choose the actin pool broadly, however, consider also checking the column fractions by dot blot with mAB 3F2 to identify capping protein. Pool the actin fractions
Isolation and Culture of Human Airway Smooth Muscle Cells
doublings and express smooth muscle cell-specific markers such as myosin heavy chain, myosin light chain kinase, α-actin, caldesmon, and SM22α . In addition, the synthetic function of these cells appears to be comparable to those studied in vivo
. For primary antibody use anti-GST at 1:1000 and anti-actin at 1:1000. For secondary use HRP conjugated IgG at 1:10,000. Actin Capture Assay Reagants Binding Buffer: 1X PBS pH 7.2 (Lane Recipe) + 10% Glycerol + 1mMMgCl2 + 0.2mM ATP + 0.1% BSA
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