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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
A synthesized peptide derived from human Cyclin E1
- 形态:
liquid
- 保存条件:
Store at 4˚C
- 克隆性:
多克隆
- 标记物:
AF350: 346nm/442nm <br/>AF405: 401nm/421nm <br/>AF488: 493nm/519nm <br/>AF555: 555nm/565nm <br/>AF594: 591nm/614nm <br/>AF647: 651nm/667nm <br/>AF680: 679nm/702nm <br/>AF750: 749nm/775nm <br/>Biotin
- 适应物种:
Hu
- 保质期:
6 months
- 库存:
详询
- 宿主:
Rabbit
- 抗体英文名:
Cyclin E1 Conjugated Antibody
- 规格:
100ul
Suggested Dilution:
AF350 conjugated: most applications: 1: 50 - 1: 250
AF405 conjugated: most applications: 1: 50 - 1: 250
AF488 conjugated: most applications: 1: 50 - 1: 250
AF555 conjugated: most applications: 1: 50 - 1: 250
AF594 conjugated: most applications: 1: 50 - 1: 250
AF647 conjugated: most applications: 1: 50 - 1: 250
AF680 conjugated: most applications: 1: 50 - 1: 250
AF750 conjugated: most applications: 1: 50 - 1: 250
Biotin conjugated: working with enzyme-conjugated streptavidin,most applications: 1: 50 - 1: 1,000
specificity:
Cyclin E1 Antibody detects endogenous levels of total Cyclin E1
accession_no:
Swiss-Prot#:
NCBI Gene ID:
NCBI mRNA#:BC027881
NCBI Protein#:formulation:
0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 10mg/ml Bovine Serum Albumin, 0.05% Sodium Azide
产品详细信息请点击:Cyclin E1 Conjugated Antibody
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文献和实验Fluoroimmunoassays Using Antibody-Conjugated Quantum Dots
between negatively charged dihydrolipoic acid (DHLA)-capped CdSe-ZnS core-shell quantum dots and positively charged proteins (natural or engineered) that serve to bridge the quantum dot and antibody. This chapter details the materials and methods for synthesis
microRNA-195通过直接影响靶基因Cyclin D1和Cyclin E1的表达 抑制了人神经胶质瘤细胞的增殖
膜母细胞瘤(pRb)和增殖细胞核抗原(PCNA)的形成。相反,抑制了miR-195的作用后,神经胶质瘤细胞的增殖速度加快,S期细胞的比例升高,G1/G0期的细胞数减少,提高细胞的非依赖型锚定生长能力,促进细胞中pRb和PCNA的磷酸化。此外,我们还发现miR-195通过直接作用于Cyclin D1和Cyclin E1的mRNA 3’UTR区下调Cyclin D1’和Cyclin E1的表达,抑制了神经胶质瘤细胞的增殖。综上所述,该实验结果表明miR-195在抑制神经胶质瘤细胞增殖的过程中发挥了重要的作用,并阐明
Immunodetection of cyclin D1 and D2/D3 using flow cytometry
that has been kept -20°C. Incubate the cells for at least two hours. Wash once with staining buffer and decant. Add the primary anti-cyclin antibody in a volume of 200 µ l. Incubate overnight at 4°C. Wash twice with cold cyclin staining buffer and add the Cy
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