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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
The antiserum was produced against synthesized non-phosphopeptide derived from human Cyclin E1 around the phosphorylation site of threonine 395 (L-L-TP-P-P).
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Polyclonal
- 适应物种:
Human
- 保质期:
12 months
- 抗原来源:
Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
IF
- 靶点:
Uniprot:P24864
- 抗体英文名:
Cyclin E1 Conjugated Antibody
- 规格:
50ul/100ul
配方:0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
应用详情:IF:1:50-1:200
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文献和实验microRNA-195通过直接影响靶基因Cyclin D1和Cyclin E1的表达 抑制了人神经胶质瘤细胞的增殖
膜母细胞瘤(pRb)和增殖细胞核抗原(PCNA)的形成。相反,抑制了miR-195的作用后,神经胶质瘤细胞的增殖速度加快,S期细胞的比例升高,G1/G0期的细胞数减少,提高细胞的非依赖型锚定生长能力,促进细胞中pRb和PCNA的磷酸化。此外,我们还发现miR-195通过直接作用于Cyclin D1和Cyclin E1的mRNA 3’UTR区下调Cyclin D1’和Cyclin E1的表达,抑制了神经胶质瘤细胞的增殖。综上所述,该实验结果表明miR-195在抑制神经胶质瘤细胞增殖的过程中发挥了重要的作用,并阐明
Immunodetection of cyclin D1 and D2/D3 using flow cytometry
that has been kept -20°C. Incubate the cells for at least two hours. Wash once with staining buffer and decant. Add the primary anti-cyclin antibody in a volume of 200 µ l. Incubate overnight at 4°C. Wash twice with cold cyclin staining buffer and add the Cy
0.1% Tween-20 and 0.2% I-Block), incubate 30 min, room temperature, gently rocking. Wash three times in 20 ml 1X Western buffer (containing 0.1% Tween-20 and 0.2% I-Block) for 5 min each. Add appropriate dilution of secondary antibody conjugated
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