- ￥2062
- LSM Bio
- 进口
- PAab00116
- 2026年03月23日
- ELISA,IHC,IP,WB,IF,FC
- E. coli - derived recombinant protein
- Human,Mouse ,Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
actin, alpha 2, smooth muscle, aorta
- 亚型：
IgG
- 形态：
liquid
- 保存条件：
负20摄氏度
- 克隆性：
Polyclonal antibody
- 标记物：
Non-conjugated
- 适应物种：
Human,Mouse ,Rat
- 保质期：
6个月
- 抗原来源：
Rabbit
- 目录编号：
Q9GZT9
- 级别：
纯化级别
- 库存：
50
- 供应商：
LSM bio
- 宿主：
E. coli - derived recombinant protein
- 应用范围：
ELISA,IHC,IP,WB,IF,FC
- 浓度：
≥95% as determined by SDS-PAGE
- 靶点：
ACTA2/smooth muscle actin
- 抗体英文名：
anti-ACTA2/smooth muscle actin antibody,ACTA2/smooth muscle actin antibody
- 抗体名：
anti-ACTA2/smooth muscle actin 抗体,ACTA2/smooth muscle actin 抗体
- 规格：
100μg
ACTA2/smooth muscle actin抗体| ACTA2/smooth muscle actin antibody
货号 PAab00116
蛋白别名 A SMA antibody, AAT6 antibody, ACTA2 antibody, ACTA2 Smooth muscle Actin antibody, Actin antibody, aortic smooth muscle antibody, Actin antibody, aortic smooth muscle Actin antibody, ACTSA antibody, ACTVS antibody, Alpha actin 2 antibody, Alpha SMA antibody, a-SMA antibody, α SMA
蛋白介绍
ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. This antibody may cross-react with other isoforms of actins.
产品描述
anti-ACTA2/smooth muscle actin antibody is a Rabbit Polyclonal antibody againstACTA2/smooth muscle actin..
建议稀释比例
WB :1:500-1:5000;IHC:1:20-1:200;IF:1:20-1:200;IP:1:500-1:5000
IHC
Immunohistochemistry of paraffin-embedded human hysteromyoma using PAab00116(ACTA2 antibody) at dilution of 1:50
Western blot
human placenta tissue were subjected to SDS PAGE followed by western blot with PAab00116(ACTA2 antibody) at dilution of 1:1000(本抗体仅供体外科研用途，不可用于临床诊断!)
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文献和实验by reaction with alkaline phosphatase-conjugated goat anti-mouse secondary antibody. Choose the actin pool broadly, however, consider also checking the column fractions by dot blot with mAB 3F2 to identify capping protein. Pool the actin fractions
LABELING MUSCLE ACTIN WITH IATR
,small vial. Procedure (perform under reduced light,4℃ unless otherwise noted) 1.Resuspend 10 mg lyophilized actin in 2 ml buffer 1.Be careful not to make bubbles. 2.Add DTT (100 mM stock)to 5 mM. 3.Dialyze against 250 ml buffer 1 overnight
Labeling Muscle Actin with 5-iodoacetamidofluorescein
with the dialysis buffer and read the OD at 495 nm. D/P = {OD495 x 41 / 60,000} / {(mg/ml)/ 43,000},should be 0.5-0.9. 3.Dilute to 3-5 mg/ml with the dialysis buffer.Calculate total mg of actin.Store as aliquots in liquid N2 after dissolving 2 mg sucrose per mg
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