产品封面图

beta-Tubulin Mouse Monoclonal

Antibody
收藏
  • ¥1100
  • Affinity
  • T0023
  • usa
  • 2025年07月09日
  • WB IHC IF
  • Mouse
  • H, R, M, Mk, Dg, Ch, Hm, Rb,Sh
  • 详细信息
  • 文献和实验
  • 技术资料
  • 抗体名

    beta-Tubulin鼠单抗(内参抗体)

  • 抗体英文名

    beta-Tubulin Mouse Monoclonal Antibody

  • 应用范围

    WB IHC IF

  • 宿主

    Mouse

  • 适应物种

    H, R, M, Mk, Dg, Ch, Hm, Rb,Sh

  • 是否单克隆

    单克隆

  • 保存条件

    -20

  • 规格

    50ul

Applications:
WB: 1:3000~1:10000, IHC: 1:200 , IF/ICC: 1:200
Reactivity:
H, R, M, Mk, Dg, Ch, Hm, Rb,Sh
Western blot analysis of A549 (1) , Rat brain (2) ,Mouse brain (3) ,Chicken lung (4) and Rabbit testis (5),Sheep muscle (6) with beta-tubulin mouse mAb.
Purification:
Affinity-chromatography
Specificity:
beta-Tubulin Mouse Monoclonal antibody detects endogenous levels of total beta-Tubulin protein.
Immunogen:
Full-length beta-tubulin protein of human
Description:
Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Tubulin may not be stable in certain cells. For example, expression of beta-Tubulin in adipose tissue is very low and therefore beta-Tubulin should not be used as loading control for these tissues.
Storage Condition and Buffer:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl

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图标文献和实验
相关实验
  • Monoclonal Antibody Production

    and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let

  • 单克隆抗体 monoclonal antibody

      指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma,在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应,对应于抗原之不同部分可制成各种不同的抗体复合体。然而,因为一个产生抗体的细胞,只能产生一种抗体,所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种,可形成边产生抗体边增殖的 hybridoma,将每个杂种细胞进行克隆(无性繁殖系)培养,则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成,这样得到的就是单克隆抗体。此抗体在测定

  • Monoclonal Antibody Production Protocol

    complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small

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