The identification of protein phosphorylation as a regulatory mechanism originated from studies by Fischer and Krebs in the mid 1950s that later earned them the 1992 Nobel prize. It is the major mechanism for the regulation of diverse cellular processes including cell division, protein synthesis, transcriptional regulation and neurotransmission. The steady state phosphorylation of any given substrate is governed by the opposing activities of kinases and phosphatases. It is now believed that a third of all eukaryotic cellular proteins are phosphorylated and that the majority of all phosphorylation events occur on serine and threonine residues (greater than 95%).
特异性
Serine-phosphorylated proteins from all species
免疫原
Phosphoserine coupled to KLH
应用
Anti-Phosphoserine Antibody, clone 4A4 is an antibody against Phosphoserine for use in WB, IF, FC, IH(P), ELISA.
Research Sub Category
General Post-translation Modification
Research Category
Signaling
质量
Routinely evaluated by immunoblot analysis on lysate from Calyculin A/Okadaic-treated human A431 carcinoma cells.
目标描述
Dependent upon the molecular weight of the serine phosphorylated protein being detected.