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- 2025年07月16日
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文献和实验into smaller pellets and processed more easily in their tubes. 4). Wash pellets with 200 mM sodium cacodylate buffer (3 x 5'). At this stage, the samples will be fixed so can be washed in any sutable buffer, and even water. Sodium cacodylate is suggested
Solid-Phase Synthesis of Neuropeptides by Fmoc Strategies
an automated peptide synthesizer that performs solid-phase peptide synthesis using the Fmoc strategy (1 ). This is based on the sequential addition of amino acid residues to an insoluble polymeric support. The base-labile Fmoc group
Immunofluorescence on ultrathin resin sections
. Residual water is drained with filter paper along the outside of the loop. Note: air-dried resin sections can be stored for months prior to labelling. Procedure: Encircle sections
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