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- 详细信息
- 文献和实验
- 技术资料
- 库存:
400
- 英文名:
ADA, Alcohol dehydrogenase>300 U/mg
- 保质期:
长期
- 供应商:
北京百奥莱博科技有限公司
- 保存条件:
-20℃
- 规格:
15kU|75kU
特别提示:包括乙醇脱氢酶(>300 U/mg)在内,本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!
产品名称:乙醇脱氢酶(>300 U/mg)
英文名称:ADA, Alcohol dehydrogenase>300 U/mg
产品货号:GS4002
产品规格:15kU|75kU
CAS号:9031-72-5
MDL号:MFCD00081305
EINECS号:232-870-4
储存条件:冷冻(-20℃)
技术规格
| 性状 | 50%甘油溶液。Yeast Supplied in 50%glycerol Containing four tightly bound zinc atoms per molecule.The optimum pH for the enzyme oxidation of ethanol is 8.6-9.0 and is closer to 7.0 for reduction of acetaldehyde.2×Crystallized.One unit reduces one micromole of NAD per minute at 25℃,pH8.8. |
我公司销售的乙醇脱氢酶(>300 U/mg)(9031-72-5),质量上佳,价格普惠,欢迎广大新老客户踊跃订购。
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文献和实验Single tube confirmation PCR protocol
containing 60 U/ml of Zymolyase (This solution is generated by resuspending 6 mg of 100T Zymolyase in 10 mls of water. Ordering information: Seikagaku Corp., #120493-1). - Sterile yellow tips work great for this task. - Repeat this step
半定量RT-PCR (Semi-Quantitative RT-PCR )
0.025 microliters 0.02 microliters Taq polymerase (protocol T.2) 14 microliters Q • Perform PCR using the following cycle parameters: 94° for 4 minutes, (94° for 30 seconds, 55° for 30 seconds, 72° for 30 seconds) x n, 72° for 5 minutes.
PIPES/sorb with 0.5 mM PMSF. If spheroplasting of some samples is completed earlier, add PIPES/sorb, invert and place on ice until all are complete. Spin down immediately--5 K, 5 minutes, 4°C. Drain. WASH SPHEROPLASTS (3x). All manipulations
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