- 询价
- ReproCell
- RCHEFM001
- 日本
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
武汉瀚湖细胞有限公司
- 英文名：
Freezing medium for human ES/iPS cells
- 库存：
大量
- 规格：
25ml
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文献和实验Neural Differentiation of Human ES and iPS Cells in Three-Dimensional Collagen and Martigel Gels
rosettes in N2 neural-inducing medium, then into nestin+ neural progenitors and further differentiated into TuJ1+ neurons, GFAP+ astrocytes, and O4+ oligodendrocytes. This 3D neural differentiation model, derived from human ES and iPS cells, has potential
1) Keep prepared solutions on ice. 2) Determine total cell count of cells to be frozen. (e.g. 1 X 108 ) 3) Determine number of vials to be frozen. (e.g. 10 vials at 1 X 107 ) 4) Centrifuge cell suspension. 5) Resuspend cell
Human Embryonic Stem (ES) Cell Protocols――Freezing Human ES Cells
pellet (gently) in 3 ml ES media to wash away collagenase. Spin 5 minutes at 1000 rpm. Resuspend pellet (again, gently!) in 0.25 ml Resuspension Medium per vial (This is one half the final volume required for freezing). Dropwise, add
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