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Designations: | BJ-5ta | ||
Depositors: | Geron Corporation | ||
Biosafety Level: | 1 | ||
Shipped: | frozen | ||
Medium & Serum: | See Propagation | ||
Growth Properties: | adherent | ||
Organism: | Homo sapiens | ||
Morphology: | fibroblast |
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Source: | Organ: skin, foreskin Cell Type: fibroblast immortalized with hTERT |
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Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
Restrictions: | This material requires that the Addendum for Commercial and For-Profit Organizations or the Addendum for Noncommercial and Academic Organizations be signed and returned to ATCC before shipment. The price listed above is for noncommercial and academic organizations only. Commercial and for-profit organizations should call for pricing. | ||
Applications: | TAs part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. We have also compared its karyotype, telomerase expression level, growth rate, morphology and tissue-specific markers when firstrecovered from cryopreservation with that of cells at 10+ population doublings to ensure that there is no change in these parameters and that the cells are capable of extended proliferation. The hTERT-immortalized foreskin fibroblast cell line, BJ-5ta, was derived by transfecting the BJ foreskin fibroblast cell line with the pGRN145 hTERT-expressing plasmid (ATCC MBA-141) at population doubling 58. Cells were cultured in medium containing hygromycin B until stable clones were selected [Pubmed: 9454332]. |
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Reverse Transcript: | N | ||
Antigen Expression: | fibroblast surface protein; Homo sapiens, expressed (fibroblast surface protein (FSP) was assayed by flow cytometry.) (fibroblast surface protein (FSP) was assayed by flow cytometry.) cytokeratin; Homo sapiens(cytokeratins were assayed by immunocytochemistry using a pan-cytokeratin antibody) (cytokeratins were assayed by immunocytochemistry using a pan-cytokeratin antibody) |
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DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 10,12 D13S317: 8,9 D16S539: 9,13 D5S818: 12 D7S820: 11,12 THO1: 7,8 TPOX: 10,11 vWA: 16,18 |
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Cytogenetic Analysis: | This is a diploid human cell line of male origin with a modal chromosome number of 46 that occurred in 90% of the cells counted. The sex chromosomes, X and Y are both karyotypically normal. | ||
Age: | newborn | ||
Gender: | male | ||
HeLa Markers: | N | ||
Comments: | The hTERT-immortalized foreskin fibroblast cell line, BJ-5ta, was derived by transfecting the BJ foreskin fibroblast cell line with the pGRN145 hTERT-expressing plasmid (ATCC MBA-141) at population doubling 58. Cells were cultured in medium containing hygromycin B until stable clones were selected [Pubmed: 9454332]. TAs part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. We have also compared its karyotype, telomerase expression level, growth rate, morphology and tissue-specific markers when firstrecovered from cryopreservation with that of cells at 10+ population doublings to ensure that there is no change in these parameters and that the cells are capable of extended proliferation. |
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Propagation: | ATCC complete growth medium: A 4:1 mixture of Dulbecco's medium and Medium 199 with supplements as follows : 4 parts of Dulbecco's Modified Eagle's Medium containing 4 mM L-glutamine, 4.5 g/L glucose and 1.5 g/L sodium bicarbonate 1 part of Medium 199 Supplemented with: 0.01 mg/ml hygromycin B 10% fetal bovine serum Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C Growth Conditions: Subculture when cell concentration reaches between 8 X 10(3) and 1 X 10(4) cells/cm2. |
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Subculturing: | Protocol: Volumes are given for a 75-cm2 flask; proportionally reduce or increase the amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:2 to 1:3 twice weekly Medium Renewal: every 2 to 3 days |
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Preservation: | Freeze medium: culture medium, 30%; fetal bovine serum, 60%; DMSO, 10% Storage temperature: liquid nitrogen vapor phase |
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Doubling Time: | 62 hr | ||
Related Products: | recommended serum:ATCC 30-2020 Trypsin-EDTA Solution:ATCC 30-2101 Cell culture tested DMSO:ATCC 4-X Erythrosin B vital stain solution:ATCC 30-2404 Trypan Blue vital stain solution:ATCC 30-2402 plasmid in bacteria:ATCC MBA-141 |
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References: | 47354: Bodnar AG, et al. Extension of life-span by introduction of telomerase into normal human cells. Science 279: 349-352, 1998. PubMed: 9454332 90421: Jiang XR, et al. Telomerase expression in human somatic cells does not induce changes associated with a transformed phenotype. Nat. Genet. 21: 111-114, 1999. PubMed: 9916802 |
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