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- 相关疾病：
其他疾病
- ATCC Number：
CRL-4008™
- 年限：
63 years
- 器官来源：
肾脏
- 细胞形态：
上皮样
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 生长状态：
贴壁生长
- 库存：
大量
- 组织来源：
angiomyolipoma
- 运输方式：
冻存运输
| Designations: | SV7tert PDGF tumor-1 | ||
| Depositors: | JL Arbiser | ||
| Biosafety Level: | 2 [cells containing SV40 viral DNA sequences ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial-like |
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| Source: | Organ: kidney Tissue: angiomyolipoma Disease: tuberous sclerosis Immortalization Method: introduction of telomerase into SV40-transfected angiomyolipoma cells |
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| Cellular Products: | Platelet Derived Growth Factor (PDGF-BB) [16173555 ] | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Restrictions: | This material requires that the Addendum for Commercial and For-Profit Organizations or the Addendum for Noncommercial and Academic Organizations be signed and returned to AT CC before shipment. The price listed above is for noncommercial and academic organizations only. Commerci al and for-profit organizations should call for pricing. | ||
| Isolation: | Isolation date: August 2003 | ||
| Applications: | In addition, it has been verified that no gross changes are observed in karyotype and morphology during the first 10 population doublings. As part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. The tumor-derived cells secrete over 18-fold more PDGF than pre-implantation cells, and demonstrate both autocrine transformation and epigenetic changes. CRL-4008 was derived from SV7tert (ATCC CRL-2461), a non-tumorigenic angiomyolipoma cell line immortalized with the SV40 large T antigen and human telomerase, by transduction with a retrovirus encoding PDGF-BB. The transduced cells were implanted into nude mice and formed tumors from which SV7tert PDGF tumor-1 (ATCC CRL-4008 ) was derived. |
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| Tumorigenic: | YES | ||
| Antigen Expression: | Positive for epithelial marker pan-cytokeratin (immunocytochemistry) | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 10, 12 D13S317: 8 D16S539: 9, 11 D5S818: 11, 13 D7S820: 11 THO1: 6, 9.3 TPOX: 8, 11 vWA: 17, 18 |
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| Cytogenetic Analysis: | This is a hypotetraploid cell line with many structural rearrangements, numerical losses and gains. The following eight derivatives were found to be present in low and high passage karyotypes: der(x)t(X;3)(q28;p21), der(1)t(1;17)(q10;p10), der(3)t(3;6)(p10:p10), i(8)(q10), i(12)(q10), der(13)t(13;21)(q10;q10), der(16)t(4;16)(q21;q24), add(20)(q13.3). Generally, the karyotyped passages contained the same complement of chromosome rearrangements, losses and gains. | ||
| Age: | 63 years | ||
| Gender: | female | ||
| Comments: | CRL-4008 was derived from SV7tert (ATCC CRL-2461), a non-tumorigenic angiomyolipoma cell line immortalized with the SV40 large T antigen and human telomerase, by transduction with a retrovirus encoding PDGF-BB. The transduced cells were implanted into nude mice and formed tumors from which SV7tert PDGF tumor-1 (ATCC CRL-4008 ) was derived. The tumor-derived cells secrete over 18-fold more PDGF than pre-implantation cells, and demonstrate both autocrine transformation and epigenetic changes. [16173555 ] As part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. In addition, it has been verified that no gross changes are observed in karyotype and morphology during the first 10 population doublings. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2 ), 5% |
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| Subculturing: | Protocol: Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation ratio: A subcultivation ratio of 1:4 to 1:10 is recommended. Medium renewal: Every 2 to 3 days |
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| Preservation: | Freeze medium: complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| Doubling Time: | approximately 28 hours | ||
| Related Products: | Recommended medium (without the additional serum described under ATCC Medium): ATCC 30-2002 Recommended serum: ATCC 30-2020 0.25% (w/v) Trypsin - 0.53mM EDTA in Hank's BSS (w/o Ca++, Mg ++): ATCC 30-2101 Cell culture tested DMSO: ATCC 4-X Phosphate-buffered saline: ATCC 30-2200 Parental cell line: ATCC CRL-2461 |
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| References: | 47354: Bodnar AG, et al. Extension of life-span by introduction of telomerase into normal human cells. Science 279: 349-352, 1998. PubMed: 9454332 53507: Arbiser JL, et al. The generation and characterization of a cell line derived from a sporadic renal angiomyolipoma: use of telomerase to obtain stable populations of cells from benign neoplasms. Am. J. Pathol. 159: 483-491, 2001. PubMed: 11485907 16173555: Govindarajan B, et al. Malignant transformation of human cells by constitutive expression of platelet-derived growth factor-BB. J. Biol. Chem. 280(14): 13936-13943, 2005. PubMed: 15695519 16173562: Arbiser JL, et al. Functional tyrosine kinase inhibitor profiling: a generally applicable method points to a novel role of platelet-derived growth factor receptor-beta in tuberous sclerosis. Am. J. Pathol. 161(3): 781-786, 2002. PubMed: 12213705 |
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文献和实验相关实验
Using Retroviral Vectors to Express SV40 Tumor Antigens
Retroviral vectors for polyomavirus tumor antigens, including SV40 large and small tumor antigens (SVT), have been extensively utilized to study the function of these proteins in a wide range of cell types. Properties of retroviruses
Cytotoxic T Lymphocytes in SV40 Infections
specific for the large tumor antigen, or T antigen (T-ag) encoded by SV40. In addition, the T-ag in transformed or tumor cells also serves as the target for cytotoxic T lymphocytes (CTL) that are capable of controlling tumor development (2 ). CTL responses
Detection of SV40 DNA Sequences in Human Tissue
, the authentication of amplified products by DNA sequencing, the finding of a single 72-bp viral-enhancer repeat element, variability of large tumor antigen (T-ag) carboxy terminal DNA sequences in many specimens, and the isolation of SV40 virus from a choroid plexus
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SV7tert PDGF tumor-1
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