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- 详细信息
- 询价记录
- 技术资料
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 库存：
大量
- 年限：
36
- 细胞形态：
上皮样
- 器官来源：
肺（及支气管）
- 运输方式：
冻存运输
- ATCC Number：
CRL-4011™
- 生长状态：
贴壁生长
| Designations: | NuLi-1 | ||
| Depositors: | AJ Klingelhutz | ||
| Biosafety Level: | 2 [cells containing SV40 and HPV viral DNA sequences ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens deposited as Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: lung; bronchus | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Restrictions: | This material requires that the Addendum for Commercial and For-Profit Organizations or the Addendum for Noncommercial and Academic Organizations be signed and returned to ATCC before shipment. The price listed above is for noncommercial and academic organizations only. Commercial and for-profit organizations should call for pricing. | ||
| Isolation: | Isolation date: 2000 | ||
| Applications: | As part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. | ||
| DNA Profile (STR): | Amelogenin: X,Y | ||
| Cytogenetic Analysis: | This is a near-diploid human cell line of male origin with a polyploidy rate of 24%. There were copies of karyotypically normal X and Y-chromosomes present in most of the cells analyzed. Overall, some of the cells contained chromosomal abnormalities, with the most consistent being trisomy 5 and 20. | ||
| Age: | 36 | ||
| Gender: | male | ||
| Comments: | Human airway epithelial (HAE) cell line, NuLi-1, was derived from normal lung of a 36-year-old patient by dual retroviral infection with HPV-16E6/E7-LXSN [Pubmed: 1845902] and hTERT-LXSN [Pubmed: 9817205]. Consequently, the cells do not undergo growth arrest in cell culture due to exogenous expression of the telomerase and E6/E7 genes. NuLi-1 cells (wild type) do not express the delta F508 cystic fibrosis-causing mutation. Another hTERT-immortalized cell line, derived from HAE of a cystic fibrosis patient is also available as ATCC CRL-4013 (CuFi-1). Both cell lines, when seeded on semipermeable filters and grown at the air-liquid interface, are capable of forming polarized differentiated epithelia that exhibit transepithelial resistance and maintain the ion channel physiology expected of each genotype. These cell lines may be useful models for studying ion physiology, therapeutic interventions for cystic fibrosis and innate immunity [Pubmed: 12676769]. As part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. We have also compared its karyotype, telomerase expression level, growth rate, morphology and tissue-specific markers when first recovered from cryopreservation with that of cells at 10+ population doublings to ensure that there is no change in these parameters and that the cells are capable of extended proliferation. |
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| Propagation: | ATCC complete growth medium: These cells are grown in a serum-free medium: BEGM (Bronchial Epithelial Growth Medium, Serum-free) from Lonza (BEGM Bullet Kit; CC-3170) made of BEBM basal medium and SingleQuot additives (ATCC does not use gentamycin-amphotericin B) supplemented with 50 �g/ml G-418. Atmosphere: 5% CO2 in air recommended Temperature: 37.0°C |
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| Subculturing: | Protocol: Note: The culture flasks should be pre-coated with 60ug/ml solution of Human Placental Collagen Type IV. (Sigma Cat. No. C-7521) at least 18 hours in advance then air-dried and rinsed 2-3 times with Dulbecco?s Phosphate Buffered Saline.
Subcultivation Ratio: 1:5 Medium Renewal: Every 2-3 days (do not wait longer) |
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| Preservation: | Freeze medium: BEGM with 30% FBS and 10% DMSO Storage temperature: liquid nitrogen vapor phase |
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| Doubling Time: | about 43 hours | ||
| Related Products: | recommended serum:ATCC 30-2020 | ||
| References: | 22566: Halbert CL, et al. The E7 gene of human papillomavirus type 16 is sufficient for immortalization of human epithelial cells. J. Virol. 65: 473-478, 1991. PubMed: 1845902 47354: Bodnar AG, et al. Extension of life-span by introduction of telomerase into normal human cells. Science 279: 349-352, 1998. PubMed: 9454332 92666: Zabner J, et al. Development of cystic fibrosis and noncystic fibrosis airway cell lines . Am. J. Physiol. Lung Cell Mol Physiol. 284: L844-L854, 2003. PubMed: 12676769 92667: Kiyono T, et al. Both Rb/p161NK4a inactivation and telomerase activity are required to immortalize human epithelial cells. Nature 396: 84-88, 1998. PubMed: 9817205 |
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NuLi-1
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