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- 询价记录
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- 技术资料
- 细胞形态：
上皮样
- 器官来源：
胃
- 生长状态：
贴壁生长
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 库存：
大量
- 相关疾病：
胃癌
- 运输方式：
冻存运输
- ATCC Number：
CRL-5822™
| Designations: | NCI-N87 [N87] | ||
| Depositors: | J Park | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
||
| Source: | Organ: stomach Disease: gastric carcinoma Derived from metastatic site: liver |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Isolation date: 1976 | ||
| Receptors: | acetylcholine, muscarinic, expressed [23078 ] | ||
| Tumorigenic: | Yes | ||
| Oncogene: | myc +; erb B2 + | ||
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 8,12 D13S317: 8,11 D16S539: 9,13 D5S818: 12,13 D7S820: 10,11 THO1: 9 TPOX: 9,11 vWA: 15,16 |
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| Cytogenetic Analysis: | near diploid; DM were present in 64% of cells examined | ||
| Gender: | male | ||
| Comments: | NCI-N87 cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG 72, and are L-dopa decarboxylase (DDC) negative. They were minimally positive for vasoactive intestinal peptide (VIP) receptors and lacked gastrin receptors. They were fiound to express receptors for muscarinic cholinergic agents. No evidence of amplification or rearrangements was noted with the N-myc, L-myc, myb and EGF receptor genes. The cell line expressed levels of c-myc and c-erb-B 2 RNA that were comparable to other cell lines.There was no expression of the following genes: N-myc, L-myc, c-cis, IGF-2, or gastrin releasing peptide. NCI-N87 cells have a reported plating efficiency of 4.3%. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C Growth Conditions: They grow as an adherent monolayer of tightly knit epithelial cells. |
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| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended Medium Renewal: Two to three times weekly |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Doubling Time: | 47 hrs | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 |
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| References: | 23078: Park JG, et al. Characteristics of cell lines established from human gastric carcinoma. Cancer Res. 50: 2773-2780, 1990. PubMed: 2158397 23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.. |
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