- 询价
- 2025年12月29日
15 年金牌会员
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 年限：
33 years
- 生长状态：
悬浮生长
- 相关疾病：
大肠癌
- ATCC Number：
CCL-251™
- 器官来源：
盲肠
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 运输方式：
冻存运输
- 细胞形态：
上皮样
- 库存：
大量
| Designations: | NCI-H716 [H716] | ||
| Depositors: | AF Gazdar | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension, multicell aggregates and some adherent cells | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
||
| Source: | Organ: cecum Disease: colorectal adenocarcinoma |
||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Restrictions: | The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233. | ||
| Isolation: | Isolation date: 1984 | ||
| Tumorigenic: | Yes | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 11 D13S317: 8,11 D16S539: 11,12 D5S818: 11 D7S820: 10,11 THO1: 6,9.3 TPOX: 8,11 vWA: 16,17 |
||
| Cytogenetic Analysis: | modal number = 61; range = 55 to 64. This is a hypotriploid human cell line. The modal chromosome number is 61, occurring in 28% of cells. The rate of higher ploidies is 1.2%. Twelve marker chromosomes are common in these cells. These include t(7q,16q), t(5p,7p), t(4q,9q) and 9 others. A large number of DM's is found in every cell. The N21 is absent; and N9, N11, N13, N20, N22 and X are single-copied. No Y chromosome was detected in the Q-banded slide preparation. |
||
| Isoenzymes: | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 Me-2, 1 PGM1, 1 PGM3, 1-2 |
||
| Age: | 33 years | ||
| Gender: | male | ||
| Ethnicity: | Caucasian | ||
| Comments: | This line was derived from cells present in ascites fluid obtained from a patient after treatment with 5-fluorouracil. The cells contain Dopa decarboxylase and, unlike other colorectal lines, contain cytoplasmic dense core granules characteristic of endocrine secretion. The cells do not express the TAG-72 or CA19-9 antigens nor do they produce carcinoembryonic antigen (CEA). |
||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
||
| Subculturing: | Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium Cultures can be established by centrifugation with subsequent resuspension at 4 to 8 X 10(5) viable cells/ml. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: Every 2 to 3 days |
||
| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 |
||
| References: | 23066: Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249 | ||
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
文献和实验相关实验
都是贴壁细胞，在消化传代过程中，步骤如下：倒尽旧的培养液－＞用无血清的培养基清洗一两次－＞加入一定量的胰酶，置于37度培养箱中5--10分钟，使细胞悬浮－＞显微镜下观察，待细胞大部分变圆时，回到超静台－＞加入一定量的含血清的新培养液，以终止胰酶作用－＞反复吹打细胞－＞再置显微镜下观察，直到细胞全部悬浮起来－＞吸出一部分加入新的培养瓶中－＞最后再补充加入一定量新的培养液。注意： 1、吹细胞时尽量多吹边角儿，此处细胞生长的多。2、吸出细胞前要混匀，可以剧烈震荡培养瓶。3、我们用的是DMEM
Science 子刊揭露遗传性肺癌亚型，我们离肺癌精准医疗还有多远？
Medicine 2021 年 1 月 27 号，来自美国国家癌症研究所（NCI）的 Anish Thomas 教授带领团队在 Science 子刊 Science Translational Medicine 上发表了题为 Whole-exome sequencing reveals germline-mutated small cell lung cancer subtype with favorable response to DNA repair–targeted therapies 的研究
一、实验材料NCI-H1299，购自上海晶莱生物技术有限公司。表 2.1.1 主要试剂试剂与耗材厂家（货号）细胞培养瓶FALCON 中国（353014）Penicillin/streptomycin solution（KGY002）0.25% Tripsin-EDTA（BK-E3076）PBS（BK330-2）胎牛血清GIBCO 美国（10270-106）1640 培养基Hyclone 美国（SH3080901）Annexin V-FITC/PI 凋亡试剂盒 二、实验仪器表 2.2.1 主要
技术资料暂无技术资料 索取技术资料
NCI-H716
询价
