- 询价
- 2025年12月29日
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 器官来源：
肺
- 生长状态：
贴壁生长
- 库存：
大量
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 细胞形态：
上皮样
- 年限：
43 years
- 运输方式：
冻存运输
- 相关疾病：
其他疾病
- ATCC Number：
HTB-183™
| Designations: | NCI-H661 [H661] | ||
| Depositors: | AF Gazdar, JD Minna | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
||
| Source: | Organ: lung Disease: carcinoma; large cell lung cancer Derived from metastatic site: lymph node |
||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Bethesda Maryland, Isolation date: 1982 |
||
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 10 D13S317: 11 D16S539: 12 D5S818: 11 D7S820: 8,10 THO1: 8 TPOX: 8 vWA: 17 |
||
| Cytogenetic Analysis: | hyperhexaploid; modal number = 142; range = 130 to 153. Each cell had over 70 marker chromosomes. Among the markers were t(1p10q), del(1)(q11/q12), 6q+, 2q+,der(20)t(12;20)(q11;q13). Some of these had 2 to copies per cell. Most cells also had 5 to 10 microchromosomes (metacentric like). Structurally normal N2,N4 and N9 were absent; two copies of the X and multiple copies of the Y were detected. | ||
| Isoenzymes: | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 Me-2, 0 PGM1, 1 PGM3, 1 |
||
| Age: | 43 years | ||
| Gender: | male | ||
| Ethnicity: | Caucasian | ||
| Comments: | The line lacks ultrastructural and biochemical evidence of squamous differentiation or mucin production. The cells express easily detectable p53 mRNA at levels comparable to normal lung tissue, and exhibit no gross structural DNA abnormalities. The cells stain positively for keratin and vimentin but are negative for neurofilament triplet protein. |
||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
||
| Subculturing: | Protocol: Remove medium, add fresh 0.25% trypsin, 0.53 mM EDTA, rinse quickly and remove trypsin leaving 1 to 2 ml. Let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, disperse cells and transfer to a new flask. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended Medium Renewal: 2 to 3 times per week |
||
| Preservation: | Freeze medium: Culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
||
| References: | 1605: Banks-Schlegel SP, et al. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 45: 1187-1197, 1985. PubMed: 2578876 1806: Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494 22437: Levitt ML, et al. Cross-linked envelope-related markers for squamous differentiation in human lung cancer cell lines. Cancer Res. 50: 120-128, 1990. PubMed: 1967140 |
||
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
文献和实验都是贴壁细胞，在消化传代过程中，步骤如下：倒尽旧的培养液－＞用无血清的培养基清洗一两次－＞加入一定量的胰酶，置于37度培养箱中5--10分钟，使细胞悬浮－＞显微镜下观察，待细胞大部分变圆时，回到超静台－＞加入一定量的含血清的新培养液，以终止胰酶作用－＞反复吹打细胞－＞再置显微镜下观察，直到细胞全部悬浮起来－＞吸出一部分加入新的培养瓶中－＞最后再补充加入一定量新的培养液。注意： 1、吹细胞时尽量多吹边角儿，此处细胞生长的多。2、吸出细胞前要混匀，可以剧烈震荡培养瓶。3、我们用的是DMEM
Science 子刊揭露遗传性肺癌亚型，我们离肺癌精准医疗还有多远？
Medicine 2021 年 1 月 27 号，来自美国国家癌症研究所（NCI）的 Anish Thomas 教授带领团队在 Science 子刊 Science Translational Medicine 上发表了题为 Whole-exome sequencing reveals germline-mutated small cell lung cancer subtype with favorable response to DNA repair–targeted therapies 的研究
一、实验材料NCI-H1299，购自上海晶莱生物技术有限公司。表 2.1.1 主要试剂试剂与耗材厂家（货号）细胞培养瓶FALCON 中国（353014）Penicillin/streptomycin solution（KGY002）0.25% Tripsin-EDTA（BK-E3076）PBS（BK330-2）胎牛血清GIBCO 美国（10270-106）1640 培养基Hyclone 美国（SH3080901）Annexin V-FITC/PI 凋亡试剂盒 二、实验仪器表 2.2.1 主要
技术资料暂无技术资料 索取技术资料
