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- 相关疾病：
鳞状细胞癌
- ATCC Number：
HTB-182™
- 库存：
大量
- 器官来源：
肺
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 生长状态：
贴壁生长
- 细胞形态：
上皮样
- 运输方式：
冻存运输
| Designations: | NCI-H520 [H520] | ||
| Depositors: | AF Gazdar, JD Minna | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: lung Disease: squamous cell carcinoma |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host (Roche Transfection Reagents ) | ||
| Tumorigenic: | Yes | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 10 D13S317: 10,11 D16S539: 8,13 D5S818: 12,13 D7S820: 8,12 THO1: 10 TPOX: 8 vWA: 18,19 |
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| Cytogenetic Analysis: | This is a hypotriploid human cell line. The modal chromosome number is 58 occurring at 30%. The frequency of higher ploidies was 3.2%. Over 30 marker chromosomes were common to all cells, and four others were found in some cells. Among the common markers were 1q+, t(1q8q), 2q+, der(16)t(3;16)(q21;q22), der(19)t(13;19)(q21;q13), and der(5)t(5;5)(p15pq13). Generally, there were two copies of der(5) in each cell. Normal Y and D group chromosomes were absent, and the X chromosome was single. | ||
| Isoenzymes: | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 0 PGM1, 1 PGM3, 1 |
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| Gender: | male | ||
| Comments: | The NCI-H520 cell line was derived by A.F. Gazdar and associates in 1982 from a sample of a lung mass taken from a patient with squamous cell carcimoma of the lung. The cells express greatly reduced levels of p53 mRNA relative to normal lung tissue, but exhibit no gross structural DNA abnormalities. The cells stain positively for keratin and vimentin but are negative for neurofilament triplet protein. The line can be cloned in soft agar (with or without serum). |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: 2 to 3 times per week |
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| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
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| Doubling Time: | 61 hrs in medium with serum; 25 to 32 hrs in serum-free medium | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 |
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| References: | 1605: Banks-Schlegel SP, et al. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 45: 1187-1197, 1985. PubMed: 2578876 1806: Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494 22434: Brower M, et al. Growth of cell lines and clinical specimens of human non-small cell lung cancer in a serum-free defined medium. Cancer Res. 46: 798-806, 1986. PubMed: 3940644 |
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都是贴壁细胞，在消化传代过程中，步骤如下：倒尽旧的培养液－＞用无血清的培养基清洗一两次－＞加入一定量的胰酶，置于37度培养箱中5--10分钟，使细胞悬浮－＞显微镜下观察，待细胞大部分变圆时，回到超静台－＞加入一定量的含血清的新培养液，以终止胰酶作用－＞反复吹打细胞－＞再置显微镜下观察，直到细胞全部悬浮起来－＞吸出一部分加入新的培养瓶中－＞最后再补充加入一定量新的培养液。注意： 1、吹细胞时尽量多吹边角儿，此处细胞生长的多。2、吸出细胞前要混匀，可以剧烈震荡培养瓶。3、我们用的是DMEM
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