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- 技术资料
- 库存：
大量
- 年限：
13 months
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 生长状态：
贴壁生长
- 相关疾病：
神经母细胞瘤
- 器官来源：
大脑
- ATCC Number：
CCL-127™
- 细胞形态：
其他
- 运输方式：
冻存运输
| Designations: | IMR-32 | ||
| Depositors: | WW Nichols | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | fibroblast; neuroblast |
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| Source: | Organ: brain Disease: neuroblastoma derived from metastatic site: abdominal mass |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Isolation date: April, 1967 | ||
| Applications: | transfection host | ||
| Virus Resistance: | echovirus 11 | ||
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 9 D16S539: 8 D5S818: 11,12 D7S820: 9,10 THO1: 7,9.3 TPOX: 11 vWA: 15 |
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| Cytogenetic Analysis: | Stable male karyotype with stemline number of 49. Two large marker chromosomes with submedian centromeres. A deletion in one number 1 chromosome: One number 16 chromosome missing; two extra chromosomes in C group. Sublines with 50 and 48 chromosomes differ from those with 49 chromosomes by having an extra or missing C group chromosome respectively. | ||
| Isoenzymes: | G6PD, B | ||
| Age: | 13 months | ||
| Gender: | male | ||
| Ethnicity: | Caucasian | ||
| Comments: | The IMR-32 cell line was established by W.W. Nichols, J. Lee and S. Dwight in April, 1967 from an abdominal mass occurring in a 13-month-old Caucasian male. [22190 ] The tumor was diagnosed as a neuroblastoma with rare areas of organoid differentiation. Two cell types are present. Predominant is a small neuroblast-like cell. The other is a large hyaline fibroblast. The cell line was submitted to the American Type Culture Collection in the 36th passage. It has been demonstrated that the cells can be propagated successfully beyond the 80th serial subculture. CCL-127 cells may pile up and grow in patches. (Please see the photos of CCL-127 on the ATCC website at www.atcc.org). CCL-127 cells may not become 100% confluent. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.Maintain cultures at a cell concentration between 4 X 10 exp4 and 4 X 10 exp5 cells/cm2. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: Every 2 to 3 days |
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| Preservation: | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
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| Doubling Time: | approximately 20 hrs. | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 recommended serum:ATCC 30-2020 |
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| References: | 22190: Tumilowicz JJ, et al. Definition of a continuous human cell line derived from neuroblastoma. Cancer Res. 30: 2110-2118, 1970. PubMed: 5459762 32287: Rostomily RC, et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024 32459: Maestrini E, et al. A family of transmembrane proteins with homology to the MET-hepatocyte growth factor receptor. Proc. Natl. Acad. Sci. USA 93: 674-678, 1996. PubMed: 8570614 |
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IMR-32
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