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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存：
大量
- 器官来源：
肺
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 运输方式：
冻存运输
- 细胞形态：
上皮样
- 相关疾病：
其他疾病
- ATCC Number：
CRL-5807™
- 组织来源：
bronchiole; alveolus
- 生长状态：
贴壁生长
| Designations: | NCI-H358 [H-358, H358] | ||
| Depositors: | AF Gazdar, JD Minna | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: lung Tissue: bronchiole; alveolus Disease: bronchioalveolar carcinoma; non-small cell lung cancer |
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| Cellular Products: | lung surfactant associated protein A (SP-A) | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Restrictions: | The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233. | ||
| Isolation: | Isolation date: 1981 | ||
| Tumorigenic: | Yes | ||
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 8,12 D16S539: 12,13 D5S818: 10,12 D7S820: 10,11 THO1: 6 TPOX: 8,9 vWA: 17 |
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| GenoType: | EGFR (wt) [90471 ] | ||
| Gender: | male | ||
| Comments: | This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates in 1981 from tumor tissue obtained from a patient prior to initiation of chemotherapy. Ultrastructural studies demonstrated the presence of cytoplasmic structures characteristic of Clara cells. The cells expressed protein and RNA of SP-A, the major lung surfactant associated protein. SP-B and SP-C RNA was not expressed. They have a reported colony forming efficiency of 0.83% in soft agarose. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: Every 2 to 3 days |
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| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
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| Doubling Time: | 38 hrs | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 purified DNA:ATCC CRL-5807D |
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| References: | 22434: Brower M, et al. Growth of cell lines and clinical specimens of human non-small cell lung cancer in a serum-free defined medium. Cancer Res. 46: 798-806, 1986. PubMed: 3940644 23081: Gazdar AF, et al. Peripheral airway cell differentiation in human lung cancer cell lines. Cancer Res. 50: 5481-5487, 1990. PubMed: 2386953 23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.. 90471: Sordella R, et al. Gefitinib-sensitizing EGFR mutations in lung cancer activate anti-apoptotic pathways. Science 305: 1163-1167, 2004. PubMed: 15284455 |
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都是贴壁细胞，在消化传代过程中，步骤如下：倒尽旧的培养液－＞用无血清的培养基清洗一两次－＞加入一定量的胰酶，置于37度培养箱中5--10分钟，使细胞悬浮－＞显微镜下观察，待细胞大部分变圆时，回到超静台－＞加入一定量的含血清的新培养液，以终止胰酶作用－＞反复吹打细胞－＞再置显微镜下观察，直到细胞全部悬浮起来－＞吸出一部分加入新的培养瓶中－＞最后再补充加入一定量新的培养液。注意： 1、吹细胞时尽量多吹边角儿，此处细胞生长的多。2、吸出细胞前要混匀，可以剧烈震荡培养瓶。3、我们用的是DMEM
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的细胞靶点Ras 蛋白由 150 多个成员组成，包括 Ras、Rho、Rab、Arf 和 Ran 亚家族。预计 Ras-Sos 复合物可能有多种组合模式，比如 Rab-Sos、Ran-Sos、Ras、Sos 等，目前尚无法知道 Sos 可能与哪些 Ras 家族成员结合。为了确定 CHDSos-5 的结合部位，研究团队对 H358 肺癌细胞进行荧光标记，然后通过基于蛋白质组学方法来富集相互作用因子及特征。研究团队共鉴定出 143 个蛋白质靶点：K-Ras、Ran 和几种 Ras 相关蛋白（RAB
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NCI-H358
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