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- 相关疾病：
其他疾病
- ATCC Number：
CRL-1932™
- 生长状态：
贴壁生长
- 年限：
58 years
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 细胞形态：
上皮样
- 库存：
大量
- 运输方式：
冻存运输
- 器官来源：
肾脏
| Designations: | 786-O [786-0] | ||
| Depositors: | RD Williams | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: kidney Disease: renal cell adenocarcinoma |
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| Cellular Products: | parathyroid hormone (PTH) like peptide | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host | ||
| Tumorigenic: | Yes | ||
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 10 D13S317: 8 D16S539: 12 D5S818: 9 D7S820: 11,12 THO1: 6,9.3 TPOX: 8,11 vWA: 15,17 |
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| Cytogenetic Analysis: | hypertriploid; Y was present in 60% the cells examined | ||
| Age: | 58 years | ||
| Gender: | male | ||
| Ethnicity: | Caucasian | ||
| Comments: | This line was derived from a primary clear cell adenocarcinoma. The cells display both microvilli and desmosomes, and can be grown in soft agar. The cells produce a PTH like peptides that is identical to peptides produced by breast and lung tumors. The peptide has an N terminal sequence similar to PTH, has PTH like activity, and has a molecular weight of 6000 daltons. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Protocol:
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 |
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| References: | 22648: Williams RD, et al. In vitro cultivation of human renal cell cancer. I. Establishment of cells in culture. In Vitro 12: 623-627, 1976. PubMed: 1010528 22653: Williams RD, et al. In vitro cultivation of human renal cell cancer. II. Characterization of cell lines. In Vitro 14: 779-786, 1978. PubMed: 721102 23304: Thiede MA, et al. Human renal carcinoma expresses two messages encoding a parathyroid hormone-like peptide: evidence for the alternative splicing of a single- copy gene. Proc. Natl. Acad. Sci. USA 85: 4605-4609, 1988. PubMed: 3290897 |
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786-O
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