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- 技术资料
- 细胞形态：
上皮样
- 生长状态：
贴壁生长
- 器官来源：
肺
- 运输方式：
冻存运输
- 年限：
65 years adult
- 相关疾病：
非小细胞肺癌
- ATCC Number：
CCL-256™
- 库存：
大量
- 物种来源：
人
- 是否是肿瘤细胞：
1
| Designations: | NCI-H2126 [H2126] | ||
| Depositors: | AF Gazdar, JD Minna | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: lung Disease: adenocarcinoma; non-small cell lung cancer Derived from metastatic site: pleural effusion |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Restrictions: | The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233. | ||
| Tumorigenic: | Yes | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 11 D13S317: 12,14 D16S539: 12 D5S818: 11 D7S820: 8,9 THO1: 7,9.3 TPOX: 8 vWA: 17 |
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| Cytogenetic Analysis: | Modal number = 79; range = 71 to 83 This is a hypertriploid human cell line with the modal chromosome number of 79, occurring in 20% of cells. The rate of cells with a higher ploidy was 5.5%. Karyotypes were very complex. There were over 22 marker chromosomes commonly present in most cells, many with complex structural rearrangements. Among these markers were: double copies for t(10qter--10q11.2::?::13C--13qter) and der(9)t(3;9) (p12;p22?), and one copy each for del (1) (p22) and i (iq). There were two normal X chromosomes per cell. Normal chromosomes Y, N1, N5, N14, and N15 were not found. Chromosomes N20 and N22 generally had four or more copies per cell. |
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| Isoenzymes: | AK-1, 1 ES-D, 1-2 G6PD, B GLO-I, 2 Me-2, 0 PGM1, 1-2 PGM3, 2 |
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| Age: | 65 years adult | ||
| Gender: | male | ||
| Ethnicity: | Caucasian | ||
| HeLa Markers: | N | ||
| Propagation: | ATCC complete growth medium: HITES medium supplemented with 5% fetal bovine serum
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Every 2 to 3 days |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Doubling Time: | about 41 hours | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006 Medium additive:ATCC 30-2214 purified DNA:ATCC 45512 purified DNA:ATCC 45513 EBV-transformed cell line from the same patient:ATCC CCL-256.1 purified DNA:ATCC CCL-256D |
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文献和实验相关实验
一、实验材料NCI-H1299，购自上海晶莱生物技术有限公司。表 2.1.1 主要试剂试剂与耗材厂家（货号）细胞培养瓶FALCON 中国（353014）Penicillin/streptomycin solution（KGY002）0.25% Tripsin-EDTA（BK-E3076）PBS（BK330-2）胎牛血清GIBCO 美国（10270-106）1640 培养基Hyclone 美国（SH3080901）Annexin V-FITC/PI 凋亡试剂盒 二、实验仪器表 2.2.1 主要
2017 年 8 月 31 日，山东烟台毓璜顶医院呼吸内科邹慎春为第一作者和通讯作者 Yao Zhang 在《分子医学报告》（Molecular Medicine Reports）发表题为《Epicatechin induces apoptotic and autophagic cell death in NCI-H2172 non‑ small cell lung cancer cells along with concomitant mitochondrial membrane
Cancer Cell Receptor Internalization and Proliferation: Effects of Neuropeptide Analogs
into the lung cancer cell line NCI-H1299. The CPT–BB conjugate was metabolized by NCI-H1299 intracellular enzymes releasing the cytotoxic CPT. Receptor binding methods and fluorescent methods are presented for studying the internalization
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