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- 详细信息
- 询价记录
- 技术资料
- 相关疾病：
大肠癌
- ATCC Number：
CCL-231™
- 年限：
Dukes' type C, grade IV
- 库存：
大量
- 细胞形态：
上皮样
- 运输方式：
冻存运输
- 器官来源：
结肠
- 生长状态：
贴壁生长
- 物种来源：
人
- 是否是肿瘤细胞：
1
| Designations: | SW48 [SW-48] | ||
| Depositors: | A Leibovitz | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: colon Tumor Stage: Dukes' type C, grade IV Disease: colorectal adenocarcinoma |
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| Cellular Products: | carcinoembryonic antigen (CEA) 0.6 ng/10 exp6 cells/10 days; keratin | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host | ||
| Tumorigenic: | Yes | ||
| Antigen Expression: | HLA A32, A33, B27, B35; blood type AB; Rh+ | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 9, 10 D13S317: 11, 12 D16S539: 11, 13 D5S818: 10, 14 D7S820: 9, 10 THO1: 6, 9.3 TPOX: 8 vWA: 18, 20, 21 |
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| Isoenzymes: | ES-D, 1 G6PD, B PEP-D, 1 PGD, A PGM1, 1 PGM3, 1-2 |
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| Age: | 82 years | ||
| Gender: | female | ||
| Ethnicity: | Caucasian | ||
| Comments: | CSAp negative (CSAp-). The cells are positive for keratin by immunoperoxidase staining. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 100% Temperature: 37.0°C |
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| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Medium Renewal: 1 to 2 times per week |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008 recommended serum:ATCC 30-2020 |
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| References: | 22140: . . In Vitro 8: 443, 1973. 22147: Chen TR, et al. Intercellular karyotypic similarity in near-diploid cell lines of human tumor origins. Cancer Genet. Cytogenet. 10: 351-362, 1983. PubMed: 6652615 22260: Chen TR, et al. Karyotype consistency in human colorectal carcinoma cell lines established in vitro. Cancer Genet. Cytogenet. 6: 93-117, 1982. PubMed: 7104989 22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080 23025: Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501 25093: Santoro IM, Groden J. Alternative splicing of the APC gene and its association with terminal differentiation. Cancer Res. 57: 488-494, 1997. PubMed: 9012479 32265: Tsao H, et al. Novel mutations in the p16/CDKN2A binding region of the Cyclin-dependent Kinase-4 gene. Cancer Res. 58: 109-113, 1998. PubMed: 9426066 |
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SW48
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