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- 相关疾病：
大肠癌
- 器官来源：
结肠
- 库存：
大量
- 运输方式：
冻存运输
- ATCC Number：
CCL-233™
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 生长状态：
贴壁生长
- 细胞形态：
上皮样
- 年限：
Dukes' type A, grade III
| Designations: | SW1116 [SW 1116, SW-1116] | ||
| Depositors: | A Leibovitz | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: colon Tumor Stage: Dukes' type A, grade III Disease: colorectal adenocarcinoma |
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| Cellular Products: | carcinoembryonic antigen (CEA) 2654 ng/10 exp6 cells/10 days; keratin | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Tumorigenic: | Yes | ||
| Oncogene: | myc +; myb + ; ras +; fos +; sis +; p53 +; abl -; ros -; src - | ||
| Antigen Expression: | blood type O; Rh+ | ||
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 10,11 D13S317: 11,14 D16S539: 9,12 D5S818: 11,12 D7S820: 12 THO1: 6 TPOX: 8,11 vWA: 14,19 |
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| Cytogenetic Analysis: | modal number = 60; range = 50 to 62 The stemline chromosome number is hypotriploid with 2S component occurring at 2.8% and 9 markers were common to S metaphases. Neither HSR chromosomes nor DM were seen. Karyotypes were less variable between cells. |
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| Isoenzymes: | ES-D, 1 G6PD, B PEP-D, 1 PGD, A PGM1, 1 PGM3, 1-2 |
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| Age: | 73 years | ||
| Gender: | male | ||
| Ethnicity: | Caucasian | ||
| Comments: | CSAp negative (CSAp-). Colon antigen 3, negative. The cells are positive for keratin by immunoperoxidase staining. The line is positive for expression of c-myc, K-ras, H-ras, myb, sis and fos oncogenes. N-myc and N-ras oncogene expression were not detected. Tumor specific nuclear matrix proteins CC-4, CC-5 and CC-6 are expressed. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C Atmosphere: air, 100% |
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| Subculturing: | Cells must be subcultured at about 80% confuency , before they reach 90%. Protocol: 1. Remove and discard culture medium. 2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. 3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37�C to facilitate dispersal. 4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. 5. Add appropriate aliquots of the cell suspension to new culture vessels. 6. Incubate cultures at 37�C. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: 1 to 2 times per week |
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| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008 recommended serum:ATCC 30-2020 |
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| References: | 22551: Wright WC, et al. Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis. J. Natl. Cancer Inst. 66: 239-247, 1981. PubMed: 6935474 23025: Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501 23341: Keesee SK, et al. Nuclear matrix proteins in human colon cancer. Proc. Natl. Acad. Sci. USA 91: 1913-1916, 1994. PubMed: 8127905 |
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” 的概念。 1998年，Kimoto等用巢式RT-PCR方法在5种非造血系统细胞来源的肿瘤传代细胞（SW1116 肠腺癌，Hep2 喉鳞状细胞癌，MCF7 雌激素受体阳性乳腺腺癌，MDA-MB-231雌激素受体阴性乳腺腺癌，HC48 胰腺腺癌）中发现了免疫球蛋白(IgG1、IgG3、IgM、IgD、IgE、IgA)恒定区和TCRα基因的转录，认为这些肿瘤细胞中亦可能表达相应的蛋白。这为肿瘤传代细胞中可能表达Ig提供了有力的证据[15]。 同年，湖南医科大学肿瘤研究所邓锡云等从中国人鼻咽
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SW1116
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