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- 文献和实验
- 技术资料
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 相关疾病：
其他疾病
- ATCC Number：
CRL-5883™
- 年限：
stage 3B
- 运输方式：
冻存运输
- 细胞形态：
上皮样
- 器官来源：
肺
- 库存：
大量
- 生长状态：
贴壁生长
| Designations: | NCI-H1650 [H-1650, H1650] | ||
| Depositors: | AF Gazdar, JD Minna | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
||
| Source: | Organ: lung Tumor Stage: stage 3B Disease: adenocarcinoma; bronchoalveolar carcinoma Derived from metastatic site: pleural effusion |
||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Restrictions: | The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233. | ||
| Isolation: | Isolation date: May, 1987 | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 11 D13S317: 11 D16S539: 11,12 D5S818: 11 D7S820: 8,9 THO1: 9.3 TPOX: 11 vWA: 18 |
||
| GenoType: | EGFR (DelE746A750) [90471 ] | ||
| Age: | 27 years | ||
| Gender: | male | ||
| Ethnicity: | Caucasian, White | ||
| Comments: | The patient was a smoker. 10 pack years. |
||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
||
| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended Medium Renewal: Every 2 to 3 days |
||
| Preservation: | Freeze medium: Complete growth medium supplemented with 5% DMSO Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 |
||
| References: | 23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.. 90471: Sordella R, et al. Gefitinib-sensitizing EGFR mutations in lung cancer activate anti-apoptotic pathways. Science 305: 1163-1167, 2004. PubMed: 15284455 |
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2017 年 8 月 31 日，山东烟台毓璜顶医院呼吸内科邹慎春为第一作者和通讯作者 Yao Zhang 在《分子医学报告》（Molecular Medicine Reports）发表题为《Epicatechin induces apoptotic and autophagic cell death in NCI-H2172 non‑ small cell lung cancer cells along with concomitant mitochondrial membrane
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