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- 2026年01月07日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 器官来源：
骨
- 生长状态：
贴壁生长
- 细胞形态：
成纤维样
- 库存：
大量
- 相关疾病：
其他疾病
- ATCC Number：
HTB-94™
- 运输方式：
冻存运输
- 年限：
72 years
| Designations: | SW 1353 [SW 1353, SW-1353] | ||
| Depositors: | A Leibovitz | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | fibroblast |
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| Source: | Organ: bone Disease: chondrosarcoma |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host (Roche Transfection Reagents ) | ||
| Antigen Expression: | Blood type O- | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 12 D13S317: 12,13 D16S539: 11,12 D5S818: 10,11 D7S820: 9,11 THO1: 6,9 TPOX: 8,11 vWA: 16,17 |
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| Cytogenetic Analysis: | hyperdiploid 47, XX, +7; Except for the trisomic N7 no other chromosome markers are evident | ||
| Isoenzymes: | AK-1, 1 ES-D, 2 G6PD, B GLO-I, 2 PGM1, 1 PGM3, 2 |
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| Age: | 72 years | ||
| Gender: | female | ||
| Ethnicity: | Caucasian | ||
| Comments: | The SW 1353 cell line was initiated by A. Leibovitz at the Scott and White Clinic, Temple, Texas in 1977 from a primary grade II chondrosarcoma of the right humerus obtained from a 72 year old female Caucasian. The initial culture medium was L-15 containing cortisone and insulin plus 10% fetal bovine serum and antibiotics. A frozen ampule at passage 12 was received at the ATCC in January, 1982. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C Atmosphere: air, 100% |
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| Subculturing: | Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended Medium Renewal: 2 to 3 times per week |
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| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008 recommended serum:ATCC 30-2020 |
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文献和实验藤红素和 19-048 治疗后能抑制结直肠癌细胞的增殖以及降低细胞活力。为了确定雷公藤红素和 19-048 是否通过靶向 PRDX1 影响细胞氧化还原状态，作者在 SW620 和 HCT116 细胞中用 DCFH-DA 荧光探针染色检测 ROS 水平，在不同浓度的雷公藤红素和 19-048 处理后，与对照相比，细胞中的 ROS 含量急剧升高，随后检测了雷公藤红素和 19-048 对 NCM460 细胞中 ROS 水平的影响，结果表明，雷公藤红素和 19-048 对正常细胞 ROS 诱导的影响小于癌
「外泌体 + 环状 RNA」国自然双热点助力肿瘤研究发表高分文章
的外泌体能促进 CRC 的增殖、迁移和侵袭 作者从两种 CRC 细胞系（HCT116和SW480）上清中分别分离获得外泌体，经过透射电镜、NTA 和 WB 鉴定后，确认已分离出外泌体。随后，作者用外泌体处理对应的 CRC 细胞，发现外泌体能显著促进 CRC 细胞增殖、迁移和侵袭，并减少凋亡。 WB 结果也显示，外泌体能提升 CRC 细胞中 BCL-2、 N-cadherin、Vimentin、MMP9 蛋白水平，和降低 E-cadherin、Cleaved-caspase3、Cleaved
细胞，在细胞中进行病毒的包装，包装好的假病毒颗粒分泌到细胞外的培养基中，收取培养基离心后可直接用于感染宿主细胞，当目的基因进入细胞后被整合到宿主基因组，从而高效表达目的基因。慢病毒的浓缩与纯化技术方法一：超速离心沉淀法1. 取6个Ultra-clear SW28离心管，用70%乙醇消毒后，放在超净工作台中打开紫外灯继续消毒30分钟。2. 每个Ultra-clear SW28离心管中加入约32ml的预先处理的病毒上清液。3. 取一支10ml的移液管，吸取12 ml 20%的蔗糖溶液。将移液管一直插入到离心
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