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- 技术资料
- 库存：
大量
- 年限：
grade III/IV
- 器官来源：
肝
- 生长状态：
贴壁生长
- 细胞形态：
上皮样
- 相关疾病：
其他疾病
- ATCC Number：
CRL-2238™
- 运输方式：
冻存运输
- 物种来源：
人
- 是否是肿瘤细胞：
1
| Designations: | SNU-423 | ||
| Depositors: | J Park | ||
| Biosafety Level: | 2 [CELLS CONTAIN HEPATITIS B VIRUS ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: liver Tumor Stage: grade III/IV Disease: pleomorphic hepatocellular carcinoma |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Antigen Expression: | Blood Type B; Rh + | ||
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 10,13 D16S539: 9 D5S818: 10 D7S820: 12 THO1: 6,9 TPOX: 8 vWA: 15 |
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| Cytogenetic Analysis: | aneuploid; modal number = 79 | ||
| Age: | 40 years | ||
| Gender: | male | ||
| Ethnicity: | Asian | ||
| Comments: | SNU-423 was derived in 1990 by J.-G. Park and associates from a primary hepatocellular carcinoma taken from a Korean patient who had been treated by transcatheter arterial embolization with lipoidol plus doxorubicin. Tumor cells were initially cultured in ACL-4 medium supplemented with 5% heat-inactivated fetal bovine serum. After establishment, cultures were maintained in RPMI 1640 supplemented with 10% heat-inactivated fetal bovine serum. Grossly, the original tumor was single nodular with perinodular extensions. Histologically, it was trabecular type. The cultured cells are multinucleated. Hepatitis B virus (HBV) DNA was detected by Southern blot hybridization. HBV genomic RNA was not expressed. |
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| Propagation: | ATCC complete growth medium: RPMI 1640 medium, 90%; heat-inactivated fetal bovine serum, 10% |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended Medium Renewal: Every 2 to 3 days Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Add fresh trypsin solution (1 to 2 ml) and let the culture sit at room temperature (or at 37C) until the cells detach. |
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| Preservation: | Culture medium, 95%; DMSO, 5% | ||
| Doubling Time: | 72 hrs | ||
| References: | 22872: Park JG, et al. Characterization of cell lines established from human hepatocellular carcinoma. Int. J. Cancer 62: 276-282, 1995. PubMed: 7543080 | ||
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文献和实验Science：减肥原来这么简单！只要控制在白天进食，连脂肪细胞都在帮你减肥……
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