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AJE 润色服务限时 8 折起，权威机构信任 15+ 年Cytoscape 教程来了！快速实现顶刊同款通路网络图五大应用案例：Mustang Q 膜层析应用全解析1 个小工具，一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 年限：
grade III/IV
- 器官来源：
肝
- 生长状态：
贴壁生长
- 细胞形态：
上皮样
- 相关疾病：
其他疾病
- ATCC Number：
CRL-2238™
- 运输方式：
冻存运输
- 物种来源：
人
- 是否是肿瘤细胞：
1
| Designations: | SNU-423 | ||
| Depositors: | J Park | ||
| Biosafety Level: | 2 [CELLS CONTAIN HEPATITIS B VIRUS ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: liver Tumor Stage: grade III/IV Disease: pleomorphic hepatocellular carcinoma |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Antigen Expression: | Blood Type B; Rh + | ||
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 10,13 D16S539: 9 D5S818: 10 D7S820: 12 THO1: 6,9 TPOX: 8 vWA: 15 |
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| Cytogenetic Analysis: | aneuploid; modal number = 79 | ||
| Age: | 40 years | ||
| Gender: | male | ||
| Ethnicity: | Asian | ||
| Comments: | SNU-423 was derived in 1990 by J.-G. Park and associates from a primary hepatocellular carcinoma taken from a Korean patient who had been treated by transcatheter arterial embolization with lipoidol plus doxorubicin. Tumor cells were initially cultured in ACL-4 medium supplemented with 5% heat-inactivated fetal bovine serum. After establishment, cultures were maintained in RPMI 1640 supplemented with 10% heat-inactivated fetal bovine serum. Grossly, the original tumor was single nodular with perinodular extensions. Histologically, it was trabecular type. The cultured cells are multinucleated. Hepatitis B virus (HBV) DNA was detected by Southern blot hybridization. HBV genomic RNA was not expressed. |
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| Propagation: | ATCC complete growth medium: RPMI 1640 medium, 90%; heat-inactivated fetal bovine serum, 10% |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended Medium Renewal: Every 2 to 3 days Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Add fresh trypsin solution (1 to 2 ml) and let the culture sit at room temperature (or at 37C) until the cells detach. |
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| Preservation: | Culture medium, 95%; DMSO, 5% | ||
| Doubling Time: | 72 hrs | ||
| References: | 22872: Park JG, et al. Characterization of cell lines established from human hepatocellular carcinoma. Int. J. Cancer 62: 276-282, 1995. PubMed: 7543080 | ||
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文献和实验近期，来自广州海洋实验室的陈凯教授，昆明医科大学口腔医学院的杨禾丰教授和胡江天教授团队在 Bioactive Materials 杂志发表了题为「miR-423-5p-enriched small extracellular vesicles drive periodontal regeneration via Sfrp2+ cell expansion」的科研论文，IF = 20.3。 研究背景： 牙周缺损（periodontal defect）是牙周炎引发的成年人牙齿脱落的主要原因
Science：减肥原来这么简单！只要控制在白天进食，连脂肪细胞都在帮你减肥……
相比，白天进食的小鼠显示出体重的增加，以及呼吸交换比节律的改变。以体重为协变量的能量消耗分析结果显示，只在夜晚进食的小鼠的总能量消耗与体重的斜率高于只在白天进食的小鼠。这些结果表明，在非活动期进食会导致体重增加，有部分原因是能量消耗的减少。 图片来源：Science Zfp423 基因敲除增加脂肪产热可改善代谢健康 为了确定增加脂肪细胞产热是否可以饮食带来的阻止体重增加和代谢健康问题，他们使用了 Zfp423 基因敲除小鼠，该基因的缺失会使得脂肪的产热作用增强。结果发现，脂肪细胞特异
人胃癌细胞(未分化) SNL-105 KATO III 人胃癌细胞 SNL-106 NCI-N87 人胃癌细胞 SNL-107 SNU-1 人胃癌细胞 SNL-173 MKN45 人低分化胃癌细胞 SNL-176 MKN74 人胃癌细胞 SNL-304 GES-1 人胃粘膜细胞 SNL-325 Hs-746T 人胃癌细胞 SNL-365 MFC 小鼠胃癌细胞 SNL
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