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- 详细信息
- 询价记录
- 技术资料
- 运输方式：
冻存运输
- 器官来源：
舌头
- 库存：
大量
- 生长状态：
贴壁生长
- 相关疾病：
鳞状细胞癌
- ATCC Number：
CRL-1624™
- 细胞形态：
上皮样
- 年限：
55 years
- 物种来源：
人
- 是否是肿瘤细胞：
1
| Designations: | SCC-4 | ||
| Depositors: | JG Rheinwald | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial-like |
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| Source: | Organ: tongue Disease: squamous cell carcinoma |
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| Cellular Products: | epidermal keratins; 40 kD keratin | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Tumorigenic: | Yes | ||
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 11 D13S317: 11,13 D16S539: 12 D5S818: 13 D7S820: 9,11 THO1: 9.3 TPOX: 8 vWA: 15,17 |
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| Age: | 55 years | ||
| Gender: | male | ||
| Comments: | SCC-4 forms colonies in semi-solid medium, and is not induced to differentiate by anchorage deprivation. Clonal growth of these cells is improved by using a 3T3 (ATCC CCL-92 ) feeder layer (see Rheinwald and Green, Cell 6:331, 1975 for methods). ATCC grows these cells on 56-X, irradiated STO cells. It is recommended that the feeder cells be plated 24 hours before use at 2 X 10(6)/T75 in order to obtain a 30% confluent monolayer. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006.To make the complete growth medium, add the following components to the base medium:
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| Subculturing: | Medium Renewal: Every 2 to 3 days Subculture before confluency. Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Inoculate new flasks at 3 X 10 exp3 cells per sq. cm. |
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| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006 recommended serum:ATCC 30-2020 feeder layer cells:ATCC 56-X |
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| References: | 23039: Rheinwald JG, Beckett MA. Tumorigenic keratinocyte lines requiring anchorage and fibroblast support cultures from human squamous cell carcinomas. Cancer Res. 41: 1657-1663, 1981. PubMed: 7214336 26113: Rheinwald JG, Beckett MA. Defective terminal differentiation in culture as a consistent and selectable character of malignant human keratinocytes. Cell 22: 629-632, 1980. PubMed: 6160916 |
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SCC-4
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