- 询价
- 2025年08月28日
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 生长状态：
贴壁生长
- 细胞形态：
上皮样
- 运输方式：
冻存运输
- 器官来源：
膀胱
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 库存：
大量
- 相关疾病：
移行细胞癌
- ATCC Number：
CRL-1749™
| Designations: | UM-UC-3 | ||
| Depositors: | HB Grossman | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
||
| Source: | Organ: urinary bladder Disease: transitional cell carcinoma |
||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Tumorigenic: | Yes | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 10,11 D13S317: 8 D16S539: 8,9 D5S818: 12 D7S820: 8,9 THO1: 6,9 TPOX: 10 vWA: 17 |
||
| Cytogenetic Analysis: | This is a hypertriploid human cell line. The modal chromosome number was 80, occurring in 42% of cells. Cells with 78 chromosomes also occurred at a high frequency. The rate of cells with higher ploidies was 2.5%. There were 30 or more marker chromosomes in each cell. They included der(1)t(1;?) (p32;?), ?t(1p5p), i(3q), t(7q14q), ?t(2p3p) and others. The X and N3 had single copy per cell, and others were generally two to three copies per cell. | ||
| Gender: | male | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
||
| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended Medium Renewal: 2 to 3 times per week |
||
| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 recommended serum:ATCC 30-2020 |
||
| References: | 25065: Bellet D, et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997. PubMed: 9012484 26153: Grossman HB, et al. Improved growth of human urothelial carcinoma cell cultures. J. Urol. 136: 953-959, 1986. PubMed: 3761468 |
||
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验【求助】文献上细胞给药剂量用10um是10um/ml还是10um/L啊？
haohuaiyong 大家好啊，我现在想用AG490处理细胞，干扰JAK-STAT3信号通路，查了相关文献，有的给药10Um,但是不知道（呵呵，我比较菜），是10um/ml还是10um/L，还请战友帮忙解答一下。 付文献截图 xdb86 人家的m是大写的M，M就是mol/L，10uM就是10×10-6mol/L。uM已经是一个浓度单位了，所以没有你说的：是10um/ml还是10um/L。
锈孢子器 aeci（ di） um 锈菌类（ Uredinales）的生殖器官之一，亦称为锈孢子堆．生于寄主植物组织内．其形状大致呈杯状，顶端突出于寄主体外而开口。其中分化的双核性孢子称为锈孢子。锈孢子为单细胞．多呈广椭圆形、卵形或稜球形，短柄上形成念珠状，表面一般具有小疣，黄褐色或无色，有数个发芽孔。具有发芽管以行发芽，进入夏孢子期或冬孢子期。锈孢子器中，对没有外壁或仅有侧丝包围，而锈孢子仅在寄主体表出现，此称为裸锈孢子器（ caeoma）。
tjmedwnn：请问25uM的氯喹是这样配的吗？？？？大家好，我做质粒转染，说明书上说转染前要加25uM的氯喹。我买的氯喹分子量是515.86，请问大家如果换算成质量这怎么计算啊，是不是25/515.86=0.048μg然后称取0.048μg加进去啊，请大家多教教我，谢谢！丁香园网友szcsyan认为：一般配成stock，浓度高一点。比如25mM。用的时候再稀释。看你买的是多重的。比如X（mg）->； X/515.86 （毫摩）容在Vμl的DMSO（一般用DMSO溶）= 25mM
技术资料暂无技术资料 索取技术资料
