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- 运输方式：
冻存运输
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 相关疾病：
其他疾病
- ATCC Number：
CRL-11730™
- 器官来源：
卵巢
- 生长状态：
贴壁生长
- 库存：
大量
- 年限：
grade 3, stage III
- 细胞形态：
上皮样
| Designations: | TOV-21G | ||
| Depositors: | University of Montreal | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens deposited as human | ||
| Morphology: | epithelial |
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| Source: | Organ: ovary Tumor Stage: grade 3, stage III Disease: primary malignant adenocarcinoma; clear cell carcinoma |
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| Cellular Products: | keratin | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | Like OV-90 (ATCC CRL-11732 ), the OV-21G (ATCC CRL-11730 ) cell line exhibits a deletion at chromosome 3p24. The TOV-112D (ATCC CRL-11731 ) cell line does not exhibit a deletion at chromosome 3p24. | ||
| Tumorigenic: | Yes | ||
| Oncogene: | p53 + (wild type) | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 13,15 D13S317: 11,12 D16S539: 10,12 D5S818: 12,13 D7S820: 12 THO1: 7,9.3 TPOX: 8,11 vWA: 17 |
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| Cytogenetic Analysis: | 47, XX, +10 [49408 ] | ||
| Age: | 62 years | ||
| Gender: | female | ||
| Comments: | This cell line was initiated in October of 1991 from a patient of French-Canadian descent with no family history of ovarian cancer. Like OV-90 (ATCC CRL-11732 ), the OV-21G (ATCC CRL-11730 ) cell line exhibits a deletion at chromosome 3p24. The TOV-112D (ATCC CRL-11731 ) cell line does not exhibit a deletion at chromosome 3p24. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is a 1:1 mixture of MCDB 105 medium containing a final concentration of 1.5 g/L sodium bicarbonate and Medium 199 containing a final concentration of 2.2 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended Medium Renewal: Every 3 to 4 days |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | recommended serum:ATCC 30-2020 | ||
| References: | 42090: Mes-Masson AM, Provencher D. Primary cultures of normal and tumoral human ovarian epithelium. US Patent 5,710,038 dated Jan 20 1998 49408: Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993 |
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文献和实验Proteomic Profiling of Ovarian Cancer Models Using TMT-LC-MS/MS
Herein, we have utilized two cellular models of epithelial ovarian cancer (EOC), where transfer of normal chromosome 18 material into the EOC cell lines TOV-112D and TOV-21G induced in vitro and in vivo suppression of tumorigenic phenotype
如果动物品系从美国Charles River 引种的C57BL/6N，则有如下一个对应关系，仅供参考：雄性：体重（g） 日龄13～15g 24～2816～18g 29～3519～21g 36～4522～24g 45～63雌性：体重（g） 日龄13～15g 24～3116～18g 32～5219～21g 53～7722～24g 78～105
until it becomes thoroughly wetted. Poke 3 widely spaced wholes in an asymmetrical pattern through the filters and the agar near the edge of the dish with a 18G or 21G needle for future orientation (see Figure 1). Place Saran wrap on benchtop. Spot out 0.5 mL puddles
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