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- 技术资料
- 年限：
Dukes' type B
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 器官来源：
结肠
- 库存：
大量
- 运输方式：
冻存运输
- 生长状态：
贴壁生长
- 相关疾病：
大肠癌
- ATCC Number：
CL-188™
- 细胞形态：
上皮样
| Designations: | LS 174T | ||
| Depositors: | Northwestern University | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: colon Tumor Stage: Dukes' type B Disease: colorectal adenocarcinoma |
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| Cellular Products: | carcinoembryonic antigen (CEA) (The production of CEA in the ATCC seed stock was 1944 ng per 10(6) cells in 10 days.) interleukin 10 (IL-10) [22511 ] interleukin 6 (IL-6) mucin |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Tumorigenic: | Yes | ||
| Oncogene: | myc +; myb + ; ras +; fos +; p53 +; sis -; abl -; ros -; src - | ||
| Antigen Expression: | serologically defined colon cancer antigen 3; Homo sapiens, expressed HLA A2, B13, B50; Blood type O |
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| DNA Profile (STR): | Amelogenin: X CSF1PO: 10, 13, 14 D13S317: 10 D16S539: 11, 13 D5S818: 11, 15 D7S820: 11 THO1: 6, 7 TPOX: 8, 9 vWA: 15, 17 |
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| Cytogenetic Analysis: | 45,X; one X chromosome missing; no other chromosomal aberrations | ||
| Age: | 58 years | ||
| Gender: | female | ||
| Ethnicity: | Caucasian | ||
| Comments: | LS 174T is a variant of LS 180 (ATCC CL-187 ) that has been maintained by using trypsin in the subculture protocol. It is more easily subcultivated than that parent line and, like LS 180, it is reported to produce large amounts of carcinoembryonic antigen (CEA). Electron microscopic studies revealed abundant microvilli and intracytoplasmic mucin vacuoles [Pubmed ID: 1262041]. They are negative for p53 antigen expression, but positive for mRNA expression. LS 174T cells stain positively for cytokeratins. The line is positive for expression of c-myc, N-myc, H-ras, N-ras. Myb, and fos oncogenes. K-ras and sis oncogene expression were not detected. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol:
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 recommended serum:ATCC 30-2020 derived from same individual:ATCC CL-187 |
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| References: | 2148: Tom BH, et al. Human colonic adenocarcinoma cells. I. Establishment and description of a new line. In Vitro 12: 180-191, 1976. PubMed: 1262041 3524: Tom BH, et al. Process of producing carcinoembryonic antigen. US Patent 4,228,236 dated Oct 14 1980 22147: Chen TR, et al. Intercellular karyotypic similarity in near-diploid cell lines of human tumor origins. Cancer Genet. Cytogenet. 10: 351-362, 1983. PubMed: 6652615 22511: Gastl GA, et al. Interleukin-10 production by human carcinoma cell lines and its relationship to interleukin-6 expression. Int. J. Cancer 55: 96-101, 1993. PubMed: 8344757 22861: Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874 32265: Tsao H, et al. Novel mutations in the p16/CDKN2A binding region of the Cyclin-dependent Kinase-4 gene. Cancer Res. 58: 109-113, 1998. PubMed: 9426066 32377: Shibata D, et al. Genomic instability in repeated sequences is an early somatic event in colorectal tumorigenesis that persists after transformation. Nat. Genet. 6: 273-281, 1994. PubMed: 8012390 32794: Kutchera W, et al. Protaglandin H synthase 2 is expressed abnormally in human colon cancer: evidence for a transcriptional effect. Proc. Natl. Acad. Sci. USA 93: 4816-4820, 1996. PubMed: 8643486 |
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