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- 2026年10月03日
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- 文献和实验
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- 运输方式：
冻存运输
- 细胞形态：
成纤维样
- 器官来源：
皮肤
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 库存：
大量
- 生长状态：
贴壁生长
- 相关疾病：
其他疾病
- ATCC Number：
CRL-7762™
| Designations: | TE 354.T | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | fibroblast |
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| Source: | Organ: skin Disease: basal cell carcinoma |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 10,14 D13S317: 11,14 D16S539: 12 D5S818: 10,13 D7S820: 10 THO1: 6 TPOX: 8,12 vWA: 14,17 |
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| Gender: | female | ||
| Comments: | The patient was sister of donor for ATCC CRL-7714. Part of the NBL Collection. Unlike other cell lines in the NBL Collection, this item has been fully accessioned by ATCC and is covered by the standard warranty. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: Every 2 to 3 days |
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| Preservation: | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 |
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文献和实验细胞治疗技术正扮演着越来越重要的角色。其中，CAR-T 细胞治疗、间充质干细胞（MSC）治疗以及 T 细胞治疗是三种备受关注的细胞治疗方法。 这些治疗方法通过采集患者体内的特定细胞，经过体外激活、修饰、扩增等步骤，再将这些具有特定功能的细胞移植回患者体内，以达到治疗疾病的目的。为了更好地理解和应用这些治疗技术，下面我们将整合并详细叙述这三种细胞治疗的流程图内容，为研究人员和临床医生提供一个清晰的操作指南。 CAR-T 细胞治疗流程 间充质干细胞治疗流程 T 细胞治疗流程
Methods and Software in NGS for TE Analysis
The recent development of next-generation sequencing (NGS) technologies allowed various authors to imagine, test, and validate new approaches for TE analysis, in their nature, type, activity, or quantity. In this chapter, we describe briefly
. In this paper, we review the history of animal studies in cardiovascular tissue engineering and the uses of animal tissue as sources for tissue engineering. 点击下面的链接下载全文： 各种动物在TE中的应用 animal models.pdf
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