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Detroit 562

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  • 2026年01月09日
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    • 物种来源

    • 是否是肿瘤细胞

      1

    • 器官来源

    • 相关疾病

      肿瘤

    • ATCC Number

      CCL-138™

    • 库存

      大量

    • 生长状态

      贴壁生长

    • 年限

      adult

    • 细胞形态

      上皮样

    • 运输方式

      冻存运输

    Designations: Detroit 562
    Depositors:  CS Stulberg
    Biosafety Level: 1
    Shipped: frozen
    Medium & Serum: See Propagation
    Growth Properties: adherent
    Organism: Homo sapiens
    Morphology: epithelial

    Source: Organ: pharynx
    Disease: carcinoma
    Derived from metastatic site: pleural effusion
    Cellular Products: keratin
    Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
    Virus Susceptibility: Human poliovirus 1
    Vesicular stomatitis virus
    DNA Profile (STR): Amelogenin: X
    CSF1PO: 11,13
    D13S317: 12
    D16S539: 11
    D5S818: 11,12
    D7S820: 8,10
    THO1: 8,9
    TPOX: 8,10
    vWA: 16
    Cytogenetic Analysis: Modal number = 64; range = 58 to128
    A large subterminal marker chromosome, arm ratio 3:4, is found in 94% of the cells karyotyped. Five to 6 minute chromosomes are present in each cell.
    Isoenzymes: G6PD, B
    Age: adult
    Gender: female
    Ethnicity: Caucasian
    Comments: The cells are positive for keratin by immunoperoxidase staining.
    Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37.0°C
    Subculturing: Protocol:
    1. Remove and discard culture medium.
    2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
    3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
      Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37�C to facilitate dispersal.
    4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
    5. Add appropriate aliquots of the cell suspension to new culture vessels.
    6. Incubate cultures at 37�C.

    Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
    Medium Renewal: Every 2 to 3 days
    Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
    Storage temperature: liquid nitrogen vapor phase
    Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
    recommended serum:ATCC 30-2020
    References: 1071: Peterson WD Jr., et al. Glucose-6-phosphate dehydrogenase isoenzymes in human cell cultures determined by sucrose-agar gel and cellulose acetate zymograms. Proc. Soc. Exp. Biol. Med. 128: 772-776, 1968. PubMed: 5668122
    1072: Peterson WD Jr., et al. A permanent heteroploid human cell line with type B glucose-6-phosphate dehydrogenase. Proc. Soc. Exp. Biol. Med. 136: 1187-1191, 1971. PubMed: 5554463

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