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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 物种来源:
人
- 是否是肿瘤细胞:
1
- 器官来源:
咽
- 相关疾病:
肿瘤
- ATCC Number:
CCL-138™
- 库存:
大量
- 生长状态:
贴壁生长
- 年限:
adult
- 细胞形态:
上皮样
- 运输方式:
冻存运输
| Designations: | Detroit 562 | ||
| Depositors: | CS Stulberg | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: pharynx Disease: carcinoma Derived from metastatic site: pleural effusion |
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| Cellular Products: | keratin | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Virus Susceptibility: | Human poliovirus 1 Vesicular stomatitis virus |
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| DNA Profile (STR): | Amelogenin: X CSF1PO: 11,13 D13S317: 12 D16S539: 11 D5S818: 11,12 D7S820: 8,10 THO1: 8,9 TPOX: 8,10 vWA: 16 |
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| Cytogenetic Analysis: | Modal number = 64; range = 58 to128 A large subterminal marker chromosome, arm ratio 3:4, is found in 94% of the cells karyotyped. Five to 6 minute chromosomes are present in each cell. |
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| Isoenzymes: | G6PD, B | ||
| Age: | adult | ||
| Gender: | female | ||
| Ethnicity: | Caucasian | ||
| Comments: | The cells are positive for keratin by immunoperoxidase staining. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Every 2 to 3 days |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 recommended serum:ATCC 30-2020 |
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| References: | 1071: Peterson WD Jr., et al. Glucose-6-phosphate dehydrogenase isoenzymes in human cell cultures determined by sucrose-agar gel and cellulose acetate zymograms. Proc. Soc. Exp. Biol. Med. 128: 772-776, 1968. PubMed: 5668122 1072: Peterson WD Jr., et al. A permanent heteroploid human cell line with type B glucose-6-phosphate dehydrogenase. Proc. Soc. Exp. Biol. Med. 136: 1187-1191, 1971. PubMed: 5554463 |
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文献和实验The K-562 Cell Model for Analysis of Neutrophil NADPH Oxidase Function
of the phagocytic oxidase (phox system) into the promyelocytic erythroleukemia cell line, K-562. Here, we describe a series of cloned K-562 cell lines that were retrovirally transduced for stable production of one or more essential components of the phagocytic
mhy叶可 大家好,我是新手,第一次养细胞,恳请各位大虾给点关于培养K562以及K562/A02的建议。K562是悬浮细胞吗?还是半贴壁?半贴壁的话应该怎么换液?请各位老师不吝赐教,多谢,多谢。 yupingwang 悬浮细胞,离心后去上清,培养基重悬 mhy叶可 多谢,以后还要多多请教,呵呵 mhy叶可 请教楼上老师,k562/A02耐药细胞株培养时,怎么维持
of virus-specific clones using single cell sorting apparatus, permitting the determination of alloreactivity from a single TCR with known specificity. When combined with new target “detector” cells called single HLA antigen-transfected K562 cells (SALs
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