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- 文献和实验
- 技术资料
- 组织来源：
cerebellum
- 相关疾病：
其他疾病
- ATCC Number：
HTB-185™
- 运输方式：
冻存运输
- 生长状态：
悬浮生长
- 器官来源：
大脑
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 库存：
大量
- 细胞形态：
上皮样
- 年限：
6 years juvenile
| Designations: | D283 Med | ||
| Depositors: | HS Friedman | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension, multicell aggregates and some adherent cells | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: brain Tissue: cerebellum Disease: medulloblastoma Derived from metastatic site: peritoneum |
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| Cellular Products: | neurofilament proteins (NFP) | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Isolation date: 1985 | ||
| Tumorigenic: | Yes | ||
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 9,12 D13S317: 8,10 D16S539: 11 D5S818: 11 D7S820: 10 THO1: 7 TPOX: 8,11 vWA: 16,18 |
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| Cytogenetic Analysis: | The karyotype is 45, XY, -7, -8, -17, -20, der(20)t(1;20)(q12;q13), 8q+, 17p+ (range = 41 to 46). This is a hypodiploid cell line with a frequency of higher ploidies of 5.4%. Three marker chromosomes are present in all cells. They are: der(20)t(1;20)(q12;q13), 8q+ and 17p+. N7, N17 and N20 have single copies. The single X is structurally normal, and the Y chromosome is present as confirmed by fluorescence microscopy. | ||
| Isoenzymes: | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 0 PGM1, 1 PGM3, 1 |
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| Age: | 6 years juvenile | ||
| Gender: | male | ||
| Ethnicity: | Caucasian | ||
| Comments: | The D283 Med cell line was established in 1985 by Friedman et al. from malignant ascites cells and a peritoneal metastasis from a boy with medulloblastoma. The cells produce tumors in nude mice, and the resulting tumors are positive for expression of neurofibrillary proteins, glutamine synthetase and neuron specific enolase but negative for glial fibrillary acidic proteins and S100 (S-100) protein. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
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| Subculturing: | Protocol: Cultures can be maintained by addition or replacement of medium. Adherent cells can be dislodged by scraping, and cultures can be established by resuspending a cell pellet at 5 X 10(5) viable cells/ml. Maintain cultures between 4 X 10(5) and 1 X 10(6) cells/ml. Medium Renewal: 2 to 3 times per week |
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| Preservation: | Freeze medium: culture medium, 90%; DMSO, 10% Storage temperature: liquid nitrogen vapor phase |
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| Doubling Time: | 52 hrs | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 recommended serum:ATCC 30-2020 |
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| References: | 2096: Friedman HS, et al. Phenotypic and genotypic analysis of a human meduloblastoma cell line and transplantable xenograft (D341 Med) demonstrating amplification of c-myc. Am. J. Pathol. 130: 472-484, 1988. PubMed: 3279793 23092: He XM, et al. Expression of O6-methylguanine-DNA methyltransferase in six human medulloblastoma cell lines. Cancer Res. 52: 1144-1148, 1992. PubMed: 1737373 23157: Friedman HS, et al. Establishment and characterization of the human medulloblastoma cell line and transplantable xenograft D283 Med. J. Neuropathol. Exp. Neurol. 44: 592-605, 1985. PubMed: 4056828 23181: Trojanowski JQ, et al. A rapidly dividing human medulloblastoma cell line (D283 MED) expresses all three neurofilament subunits. Am. J. Pathol. 126: 358-363, 1987. PubMed: 3103453 32287: Rostomily RC, et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024 |
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