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- 2026年01月18日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 运输方式：
冻存运输
- 细胞形态：
其他
- 组织来源：
retina
- 器官来源：
眼
- 库存：
大量
- 相关疾病：
其他疾病
- ATCC Number：
HTB-169™
- 年限：
1 year
- 生长状态：
悬浮生长
- 物种来源：
人
- 是否是肿瘤细胞：
1
| Designations: | WERI-Rb-1 | ||
| Depositors: | TW Sery | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Homo sapiens | ||
| Morphology: | grape-like clusters of round cells |
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| Source: | Organ: eye Tissue: retina Disease: retinoblastoma |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Isolation date: 1974 | ||
| Applications: | transfection host | ||
| Tumorigenic: | Yes | ||
| Cytogenetic Analysis: | This is a near diploid line. The modal chromosome number is 47 occurring at 38%, and the rate of polyploidy is 9%. Fifteen to sixteen marker chromosomes are present in all cells. They are t(1,?), t(3p,5q), der(3)t(?q29;?), t(3q,?), 5q+, i(6p), t(7q,?), 9q+, t(10q,21q), 16q+ and five to six others. Normal chromosomes 3, 10, 13 and 16 are absent. There are two copies of the X chromosome. No Y chromosomes were detected in QM stained preparations | ||
| Isoenzymes: | ES-D, 1 G6PD, B GLO-I, 2 Me-2, 1 PGM1, 1 PGM3, 0 |
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| Age: | 1 year | ||
| Gender: | female | ||
| Ethnicity: | Caucasian | ||
| Comments: | The WERI-Rb-1 line is one of two human retinoblastoma cell lines established in 1974 by R.M. McFall and T.W. Sery. The cells survive culturing in Difco Bacto-Agar but do not form colonies. Scanning electron microscopy reveals some variation in the number and frequency of surface blebs, lamellipodia and microvilli. The line is of interest in studies of cell differentiation, animal models of tumor therapy and biochemical evaluations. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: 5% CO2 in air recommended Temperature: 37.0°C |
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| Subculturing: | Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 2 - 3 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 to 2 X 10 exp6 viable cells/ml. Medium Renewal: Every 3 to 4 days |
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| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 |
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| References: | 23026: McFall RC, et al. Characterization of a new continuous cell line derived from a human retinoblastoma. Cancer Res. 37: 1003-1010, 1977. PubMed: 844036 23030: McFall RC, et al. Scanning electron microscopic observation of two retinoblastoma cell lines. Cancer Res. 38: 2827-2835, 1978. PubMed: 679190 32287: Rostomily RC, et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024 |
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文献和实验feroger 看了很多文献上测p-Rb，用的是actin做的内参，没用总的Rb，这是为什么呢 kern6549 怎么能用总的Rb作为内参，看来你没有弄明白什么是内参！ feroger 测磷酸化不是要和总的对照吗？像测p-AKT还要同时测总AKT，为什么很多文献上测p-Rb，而不测总的Rb呢？ kern6549 为什么一定要和总的对照呢？ 测
Analysis of E7/Rb Associations
The product of the early gene E7 is one of the major transforming proteins of human papillomaviruses (HPVs). It exerts its activity by associating with and altering the biological functions of several cellular proteins involved in the control
CH926RB 全自动环保除湿机 型号：CH926RB 除湿量：26L/d(30℃，80%RH) 单位输入功率除湿量高于国家标准 43% 四种控湿功能 全自动控制，超宁静运转 高精度湿度感应控制系统 银离子空气滤网 压缩机保护装置 自动除霜装置 超大前置透明水箱 防溢水报警自动停机 连续除湿自动排水接口 收叠式提把及万向转轮 适用环境条件：5 ~ 35℃ 国家强制性产品认证（3C 认证） 证书
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