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- 库存：
大量
- 生长状态：
贴壁生长
- 细胞形态：
上皮样
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 器官来源：
乳房
- 运输方式：
冻存运输
- 年限：
38 years adult
- 相关疾病：
腺癌
- ATCC Number：
HTB-128™
| Designations: | MDA-MB-415 | ||
| Depositors: | R Cailleau | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: mammary gland; breast Disease: adenocarcinoma Derived from metastatic site: pleural effusion |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Isolation date: 1975 | ||
| Tumorigenic: | No | ||
| Antigen Expression: | Blood Type O | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 10,12 D13S317: 11,13 D16S539: 13 D5S818: 11,13 D7S820: 9,10 THO1: 7,9.3 TPOX: 8 vWA: 17 |
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| Cytogenetic Analysis: | modal number =73; range = 65 to 76. The stemline chromosome number is hypertriploid with the 2S component occurring at 0.8%. Ten markers (del(2), t(6;?), t(6;?), t(2;12), t(14;?), and single or paired M6, M7, M8, M9, and M10) were common to all cells, and four others (M13, M15, M16, and t(13,21) occurred in some cells. The X chromosome was generally tetrasomic and chromosome 4 was tetrasomic or pentasomic (P35). No Y chromosomes were detected in QM stained preparations. | ||
| Isoenzymes: | AK-1, 1 ES-D, 2 G6PD, A GLO-I, 1-2 Me-2, 2 PGM1, 1 PGM3, 1 |
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| Age: | 38 years adult | ||
| Gender: | female | ||
| Comments: | The cells express the WNT7B oncogene [PubMed: 8168088]. The patient presenting with the tumor was from Paraguay and, although listed as Caucasian, may have had mixed ancestry as suggested by the presence of G6PD type A phenotype in the cells. The line forms flat, spreading plaques of epithelia which exhibit desmosomes, extensive microtubules and microfilaments upon examination with the electron microscope. It is not amenable to trypsinization. |
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| Propagation: | ATCC complete growth medium: Leibovitz's L-15 medium with 2mM L-glutamine supplemented with 10 mcg/ml insulin and 10 mcg/ml glutathione, 85%; fetal bovine serum, 15% Atmosphere: air, 100% Temperature: 37.0°C |
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| Subculturing: | Protocol: Subcultures are prepared by scraping. Remove old medium, add fresh, dislodge cells, aspirate and dispense into new flasks. Subculture every 6 to 8 days. Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended Medium Renewal: 2 to 3 times per week |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008 recommended serum:ATCC 30-2020 purified DNA:ATCC 45524 purified DNA:ATCC 45525 purified DNA:ATCC HTB-128D |
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| References: | 1206: Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337 22329: Beall PT, et al. Polypeptides that inhibit human breast cancer cell division. Cancer Biochem. Biophys. 3: 93-96, 1979. PubMed: 552902 22429: Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779 22656: Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202 23113: Huguet EL, et al. Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast tissue. Cancer Res. 54: 2615-2621, 1994. PubMed: 8168088 |
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