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- 询价记录
- 文献和实验
- 技术资料
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 细胞形态：
上皮样
- 运输方式：
冻存运输
- 器官来源：
乳房
- 库存：
大量
- 相关疾病：
腺癌
- ATCC Number：
HTB-27™
- 年限：
40 years
- 生长状态：
贴壁生长
| Designations: | MDA-MB-361 | ||
| Depositors: | R Cailleau | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | loosely adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: mammary gland; breast Disease: adenocarcinoma Derived from metastatic site: brain |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Oncogene: | wnt7h + | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 12 D13S317: 11 D16S539: 11,12 D5S818: 10,11 D7S820: 9,12 THO1: 9.3 TPOX: 8,11 vWA: 17 |
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| Cytogenetic Analysis: | modal number. = 56; range = 54 to 61. The cell line is aneuploid human female, with chromosome counts in the hyperdiploid range. Normal chromosomes N11 and N17 are absent, chromosomes N1, N20, and N21 are weakly represented, and chromosomes N2, N8, N9, and N15 are single. The remainder of chromosomes are often paired. Eighteen marker chromosomes are found, of which 10 are consistently present. Some of these markers are found to be quite comparable to those described by K.L. Satya-Prakash, et al., in their report on this cell line. |
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| Isoenzymes: | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 1 PGM1, 1 PGM3, 1-2 |
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| Age: | 40 years | ||
| Gender: | female | ||
| Ethnicity: | Caucasian | ||
| Comments: | This line differs from others of the series (ATCC HTB-23 to 26) in karyology and in that it was isolated from a brain metastasis. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%. Temperature: 37.0°C Atmosphere: air, 100% |
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| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Medium Renewal: 2 to 3 times per week |
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| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008 recommended serum:ATCC 30-2020 |
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| References: | 1206: Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337 22182: Cruciger Q, et al. Morphological, biochemical and chromosomal characterization of breast tumor lines from pleural effusions. In Vitro 12: 331, 1976. 22429: Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779 22532: Cailleau R, et al. Breast tumor cell lines from pleural effusions. J. Natl. Cancer Inst. 53: 661-674, 1974. PubMed: 4412247 22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 22656: Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202 23093: Faust JB, Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. PubMed: 1568216 23113: Huguet EL, et al. Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast tissue. Cancer Res. 54: 2615-2621, 1994. PubMed: 8168088 |
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