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- 文献和实验
- 技术资料
- 器官来源:
乳房
- 年限:
35 years
- 物种来源:
人
- 是否是肿瘤细胞:
1
- 库存:
大量
- 相关疾病:
肿瘤
- ATCC Number:
CRL-7336™
- 运输方式:
冻存运输
| Designations: | Hs 566(B).T | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Organism: | Homo sapiens | ||
| Morphology: | |
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| Source: | Organ: mammary gland; breast Disease: carcinoma |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 12,13 D13S317: 12,13 D16S539: 12 D5S818: 9,13 D7S820: 8,12 THO1: 6 TPOX: 8,11 vWA: 16 |
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| Age: | 35 years | ||
| Gender: | female | ||
| Comments: | Part of the NBL Collection. Unlike other cell lines in the NBL Collection, this item has been fully accessioned by ATCC and is covered by the standard warranty. Please see the NBL Repository description. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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文献和实验1. T、B细胞相互作用的过程T细胞为Th2亚群,而B细胞既是Th细胞辅助的对象,又是T细胞活化的APC。这一相互合作包括一系列过程:①抗原以其B表位(绿色)与BCR结合传递B细胞活化的第一信号,使其C1340与细胞因子受体的表达增加,并使B细胞加工提呈抗原和表达少量D7分子;②B细胞作为APC将带有T表位(红色)的抗原肽提呈给CD4T细胞,供T细胞识别;③初步活化的T细胞表达CD40L等,与B细胞表面CD40结合成为B细胞活化的第二信号;④活化B细胞表达B7分子与CD28结合为T细胞提供第二
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with 5 ml of PBS and add to gradient. Centrifuge at 300 g for 40 minutes, room temperature. Collect the B cells at the interface. Wash 3 times with PBS. Suspend the SRBC-T cell pellet. Centrifuge at 300 d for 10 minutes. Aspirate all of the supe
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