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- 文献和实验
- 技术资料
- 生长状态：
贴壁生长
- 细胞形态：
成纤维样
- 品系：
Swiss
- 器官来源：
胚胎
- 库存：
大量
- 运输方式：
冻存运输
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 细胞类型：
成纤维细胞
- ATCC Number：
48-X™
- 年限：
embryo
| Designations: | IRR-3T3 [irradiated 3T3-Swiss albino cells] | ||
| Depositors: | H Green | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus | ||
| Morphology: | fibroblast |
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| Source: | Strain: Swiss Organ: embryo Cell Type: fibroblast |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | It is recommended that the feeder cells be plated 24 hours before use. These cells are provided to be used as feeder cells to support the growth of other cells. |
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| Reverse Transcript: | negative | ||
| Age: | embryo | ||
| Comments: | The cells have been irradiated and will not replicate. These cells are provided to be used as feeder cells to support the growth of other cells. It is recommended that the feeder cells be plated 24 hours before use. Once the feeder cells have attached, the culture medium may be changed to accommodate the cells to be supported. These cells will begin to deteriorate in 2 to 3 weeks after plating. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: bovine calf serum to a final concentration of 10%. |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2030 source culture:ATCC CCL-92 |
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| References: | 5732: Todaro GJ, Green H. Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines. J. Cell Biol. 17: 299-313, 1963. PubMed: 13985244 | ||
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文献和实验Assessment of the Expression of Prostaglandin Synthase-2 in Swiss 3T3 Fibroblasts
methods used for the detection of PGS2 expression by western and northern analyses in murine Swiss 3T3 fibroblasts. These methods have also been used successfully to detect PGS2 expression in a number of other murine cell lines (Table 1 ).
Subcellular Fractionation of Adipocytes and 3T3-L1 Cells
for the study of adipose tissue (AT) metabolism is the 3T3-L1 cell line, which differentiates to an adipocyte-like phenotype. In these cultured cells, long-term effects of hormones and agents can be investigated. As in fat cells, the effects of insulin
The cytotoxic effect of chemicals on Balb/c 3T3 cells in culture is measured by cell viability (Neutral Red Uptake) and total cell protein (Kenacid Blue R dye binding method). Healthy Balb/c 3T3 cells (an established cell line
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