I-10产品图

I-10

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  • 2026年11月13日
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    • 相关疾病:

      其他疾病

    • 运输方式:

      冻存运输

    • ATCC Number:

      CCL-83™

    • 库存:

      大量

    • 生长状态:

      贴壁生长

    • 物种来源:

      小鼠

    • 是否是肿瘤细胞:

      0

    • 器官来源:

      睾丸

    • 细胞形态:

      上皮样

    • 品系:

      BALB/cJ

    Designations: I-10
    Depositors:  G Sato
    Biosafety Level: 1
    Shipped: frozen
    Medium & Serum: See Propagation
    Growth Properties: adherent
    Organism: Mus musculus
    Morphology: epithelial

    Source: Strain: BALB/cJ
    Organ: testis
    Disease: Leydig cell tumor
    Cellular Products: 4-pregnen-3-one(20-hydroxy); progesterone
    Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
    Virus Resistance: poliovirus 1
    Antigen Expression: H-2d
    Gender: male
    Comments: Tested and found negative for ectromelia virus (mousepox).
    Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 2.5%; horse serum to a final concentration of 15%.
    Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
    Medium Renewal: Twice per week
    Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
    1. Remove and discard culture medium.
    2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
    3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
      Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
    4. Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting
    5. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
    6. Incubate cultures at 37C.
    Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%
    References: 879: Yasamura Y, et al. Establishment of four functional, clonal strains of animal cells in culture. Science 154: 1186-1189, 1966. PubMed: 4288399
    26250: Shin SI. Studies on interstitial cells in tissue culture: steroid biosynthesis in monolayers of mouse testicular interstitial cells. Endocrinology 81: 440-448, 1967. PubMed: 4382264

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