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- 详细信息
- 技术资料
- 运输方式：
冻存运输
- 相关疾病：
腺癌
- ATCC Number：
CRL-5882™
- 年限：
stage 3A
- 器官来源：
肺
- 库存：
大量
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 生长状态：
贴壁生长
| Designations: | NCI-H1648 [H1648] | ||
| Depositors: | AF Gazdar, JD Minna | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | |
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| Source: | Organ: lung Tumor Stage: stage 3A Disease: adenocarcinoma Derived from metastatic site: lymph node |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Restrictions: | The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233. | ||
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 10,12 D13S317: 12 D16S539: 11 D5S818: 11 D7S820: 10,11 THO1: 7,9.3 TPOX: 8,11 vWA: 14,17 |
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| Age: | 39 years | ||
| Gender: | male | ||
| Ethnicity: | Black | ||
| Comments: | The line was established in May 1987. The patient was a smoker. 40 pack years. A lymphoblastoid line from the same patient is available as ATCC CRL-5954. |
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| Propagation: | ATCC complete growth medium: ACL-4 medium (serum-free) The base medium for this cell line is ATCC formulated :DMEM: F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium: |
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| Subculturing: | Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: Subcultivation Ratio: 1:3 to 1:6 |
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| Preservation: | Culture medium, 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006 normal (or near-normal) cell line established from the same patient:ATCC CRL-5954 |
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| References: | 23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.. | ||
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NCI-H1648 [H1648]
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