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- 2026年02月20日
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- 细胞形态：
其他
- 库存：
大量
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 细胞类型：
其他细胞类型
- 运输方式：
冻存运输
- 器官来源：
大脑
- 相关疾病：
神经母细胞瘤
- ATCC Number：
CCL-147™
- 生长状态：
贴壁，多细胞聚集
| Designations: | NB41A3 | ||
| Depositors: | G Augusti-Tocco | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | loosely adherent, multicell aggregates | ||
| Organism: | Mus musculus | ||
| Morphology: | neuroblast; with elongated processes which emanate |
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| Source: | Organ: brain Disease: neuroblastoma Cell Type: neuroblast; |
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| Cellular Products: | choline acetylase; tyrosine hydroxylase; acetylcholinesterase | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Virus Resistance: | poliovirus 1 | ||
| Comments: | Tested and found negative for ectromelia virus (mousepox). | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 2.5%; horse serum to a final concentration of 15%. Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
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| Subculturing: | Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 to 3 times per week |
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| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004 recommended serum:ATCC 30-2020 recommended serum:ATCC 30-2040 |
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| References: | 23251: Buonassisi V, et al. Hormone-producing cultures of adrenal and pituitary tumor origin. Proc. Natl. Acad. Sci. USA 48: 1184-1190, 1962. PubMed: 13874682 26010: Burn JH, Rand MJ. Acetylcholine in adrenergic transmission. Annu. Rev. Pharmacol. 5: 163-182, 1965. PubMed: 14290806 |
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文献和实验本人做 NB 实验有那么一段时时时间了，之前苦于量化 NB 图无门道，走过不少弯路。目前虽软件多多，但无几个简单粗暴有效易上手合我心意的软件，直到偶然机会遇见了IJ 软件 Image J（本人喜欢叫它 IJ 软件 ）。下面为本人总结的量化 NB 图教程（如有错漏，请各位同行大佬批评指正）1. 启动你的大 IJ，打开一张 NB 图，强迫症的我向右拉伸对其一下 NB 图，就出现了左边黑色条条了。 2. 然后将 NB 图转为 8-bit 的灰度图，路径：Image-Type-8bit（把彩色去掉
before start: thaw cells from liquid nitrogen, grow in 75cc flask (T75) in Fischer's medium MM (maintenance medium) until content gets cloudy and starts to turn yellow (usually want ~ 6 x 106 cells per T75), assay will be performed
Studying NB-LRR Immune Receptor Localization by Agroinfiltration Transient Expression
NB-LRR immune receptors in plants play dual roles as sentries and as activators of defense. The site in the cell where these activities take place can be different for different NB-LRRs. Furthermore, recognition and defense activation
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