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- 生长状态：
悬浮生长，少量贴壁
- 库存：
大量
- 免疫类型：
IgG2a kappa
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 细胞形态：
淋巴样
- 运输方式：
冻存运输
- ATCC Number：
HB-11642™
| Designations: | ER4.7G.11 [7G11] | ||
| Depositors: | Repligen Corporation | ||
| Isotype: | IgG2a kappa | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension, with some loosely adherent cells | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: lymphoblast B lymphocyte; somatic cell hybrid | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Animals were immunized with recombinant human CTLA4. Spleen cells were fused with?Monoclonal antibody ER4.7G11 stimulates CD8 positive T cells to proliferate [U.S. Pat. 5,858,358]. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium: Temperature: 37.0°C |
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| Subculturing: | Protocol: Cultures can be maintained by addition of fresh medium. Shake off attached cells. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 10(5) viable cells/ml. Maintain cultures at a cell concentration between 1 X 10(5) and 1 X 10(6) cells/ml. Do not allow the cell concentration to exceed 1 X 10(6) cells/ml. Medium Renewal: Two to three times weekly |
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| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | recommended serum:ATCC 30-2020 Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X |
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| References: | 70728: June CH, et al. Methods for selectively stimulating proliferation of T cells. US Patent 5,858,358 dated Jan 12 1999 70730: Gray GS. CD9 antigen peptides and antibodies thereto. US Patent 5,883,223 dated Mar 16 1999 70733: June CH, et al. Methods for inducing a population of T cells to proliferate using agents which recognize TCR/CD3 and ligands which stimulate an accessory molecule on the surface of the T cells. US Patent 6,352,694 dated Mar 5 2002 70734: June CH, et al. Methods for selectively stimulating proliferation of T cells. US Patent 6,534,055 dated Mar 18 2003 |
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