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- 详细信息
- 技术资料
- 库存：
大量
- 生长状态：
贴壁生长
- 器官来源：
胰腺
- 运输方式：
冻存运输
- 年限：
12 month old
- 相关疾病：
腺癌
- ATCC Number：
CRL-2389™
- 细胞形态：
上皮样
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 品系：
LT/Sv
| Designations: | LTPA | ||
| Depositors: | EH Leiter | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus deposited as Mus musculus (mouse) | ||
| Morphology: | epithelial |
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| Source: | Organ: pancreas Strain: LT/Sv Disease: adenocarcinoma |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Cytogenetic Analysis: | Number of cells examined = 59; Modal Chromosome Number = 75 with a range of 65 to 79; Polyploidy Rate = 22% | ||
| Age: | 12 month old | ||
| Gender: | female | ||
| Comments: | LTPA is an epithelial cell line derived in 1975 by Edward H. Leiter at The Jackson Laboratory, Bar Harbor, Maine from a spontaneous pancreatic adenocarcinoma taken from a 12 month old female Lt/Sv mouse. A defective Polyoma virus infection of a pancreatic duct cell or its precursor appears to represent the neoplastic transforming event. LTPA cells carry a persistent Polyoma infection. When injected subcutaneously into Swiss nu/nu mice, LTPA cells formed ductular structures, which were destroyed by inflammatory reactions within 3 weeks. A culture submitted to the ATCC in May of 1998 was found to be contaminated with mycoplasma and progeny were cured by a 21-day treatment with BM Cycline. The cells were assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests were negative. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended Medium Renewal: Every 2 to 3 days Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. |
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| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 recommended serum:ATCC 30-2020 |
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| References: | 38749: Leiter EH, et al. An epithelial cell line with chronic polyoma infection established from a spontaneous mouse pancreatic adenocarcinoma. Cancer Res. 38: 969-977, 1978. PubMed: 205354 | ||
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LTPA
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