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- 详细信息
- 文献和实验
- 技术资料
- 年限：
19 years
- 库存：
大量
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 组织来源：
retinal pigmented epithelium; retina
- 细胞类型：
其他细胞类型
- 运输方式：
冻存运输
- 细胞形态：
上皮样
- ATCC Number：
CRL-2502™
- 生长状态：
贴壁生长
- 器官来源：
眼
| Designations: | ARPE-19/HPV-16 | ||
| Depositors: | JW Obringer | ||
| Biosafety Level: | 2 [Cells contain Human Papilloma viral 16 (HPV16) sequences ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens deposited as human | ||
| Morphology: | epithelial |
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| Source: | Organ: eye Tissue: retinal pigmented epithelium; retina Cell Type: human papillomavirus 16 (HPV-16) transfected |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host (Roche Transfection Reagents ) | ||
| Antigen Expression: | RPE-specific markers CRALBP and RPE-65 [34497 ] | ||
| DNA Profile (STR): | Amelogenin: X, Y CSF1PO: 11 D13S317: 11, 12 D16S539: 9, 11 D5S818: 13 D7S820: 9, 11 THO1: 6, 9.3 TPOX: 9, 11 vWA: 16, 19 |
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| Cytogenetic Analysis: | diploid | ||
| Age: | 19 years | ||
| Gender: | male | ||
| Comments: | The ARPE/HPV-16 transformed cell line was derived from the ARPE-19 cell line (ATCC CRL-2302 ) by transfection with DH5-HPV-16. ARPE-19 is a spontaneously arising retinal pigment epithelia (RPE) cell line derived in 1986 by Amy Aotaki-Keen from the normal eyes of a 19-year-old male who died from head trauma in a motor vehicle accident. [34497 ] The cells express the RPE-specific markers CRALBP and RPE-65. [34497 ] |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended Medium Renewal: Every 2 to 3 days Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
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| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | recommended serum:ATCC 30-2020 parental cell line:ATCC CRL-2302 |
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| References: | 34497: Dunn KC, et al. ARPE-19, A human retinal pigment epithelial cell line with differentiated properties. Exp. Eye Res. 62: 155-169, 1996. PubMed: 8698076 | ||
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文献和实验HPV 并不是宫颈癌唯一的「元凶」？这种微生物化身「帮凶」，共同促进癌症发生
，同时进行了蛋白质的表达。 为了系统评估 hpv16 E6E7 整合和随后与沙眼梭菌合并感染对宿主调节的影响，研究人员从健康志愿者上分离出宫颈外干细胞，将其嵌入基质胶中培养 3D 类器官，并检测了来自各种 HPV 类型的 E6E7 基因。来自三个供体的子宫颈外器官检测 HPV 的 E6E7 癌基因均为阴性。这些来自健康供体的 HPV E6E7 阴性的类器官被用于研究 HPV 整合对健康宫颈组织的影响。 图片来源：Nature Communications 随后，研究人员用携带 HPV16 E6
Analysis of E7/Rb Associations
of fundamental events, such as cell proliferation and apoptosis. The best-characterized activity of E7 from HPV type 16, the most frequently detected type in cervical cancer, is its ability to bind and induce degradation of the tumor-suppressor retinoblastoma
PCR引物设计和优化(PCR PRIMER DESIGN AND REACTION OPTIMISATION)
, or unless they are unusually long.An illustration of the effect of annealing temperature on the specificity and on the yield of amplification of Human papillomavirus type 16 （HPV-16） is given below （Williamson and Rybicki, 1991: J Med Virol 33: 165-171）。Plasmid and biopsy
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