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- 技术资料
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 库存：
大量
- ATCC Number：
HB-9081™
- 运输方式：
冻存运输
- 免疫类型：
IgM
- 细胞形态：
淋巴样
- 生长状态：
悬浮生长
| Designations: | XMMEN-OE5 [AAB-647, AAB647] | ||
| Depositors: | Regents of the University of California | ||
| Isotype: | IgM | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against lipopolysaccharide endotoxin of gram negative bacteria | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Tumorigenic: | Yes | ||
| Comments: | Animals were immunized with killed E. coli (0111 strain, J5 mutant) cells. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody neutralizes gram negative endotoxin activity, and is protective against gram negative sepsis and therapeutic for gram negative sepsis in mice and humans. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| Preservation: | culture medium, 90%; DMSO, 10% | ||
| References: | 4006: Young LS. Monoclonal antibodies specific for lipid-A determinants of gram negative bacteria. US Patent 4,918,163 dated Apr 17 1990 | ||
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XMMEN-OE5 [AAB-647, AAB647]
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