Disease: leukemia; lymphoma Strain: BALB/c Cell Type: B lymphocyte;
Cellular Products:
immunoglobulin (surface, sIg +); monoclonal antibody
Permits/Forms:
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Gender:
female
Comments:
The cells can be activated by LPS to produce IgM and can be used as a target for B cell growth factor (BCGF) assays. Tested and found negative for ectromelia virus (mousepox).
ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine and 0.05 mM 2-mercaptoethanol, 85%; fetal bovine serum, 15%
Subculturing:
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Every 2 to 3 days Subcultures are prepared by scraping. Dislodge cells in the spent medium, aspirate, add fresh medium and dispense into new flasks.
References:
22831: Gronowicz ES, et al. An in vitro line of the B cell tumor BCL1 can be activated by LPS to secrete IgM1. J. Immunol. 125: 976-980, 1980. PubMed: 6157730 22835: Thoman ML, Weigle WO. Identification and partial purification of a B cell growth-promoting material utilizing the BCL1 cell line. J. Immunol. 130: 233-236, 1983. PubMed: 6600179 22845: Strober S, et al. Immunobiology of a spontaneous murine B cell leukemia (BCL1). Immunol. Rev. 48: 169-195, 1979. PubMed: 121100 22904: Slavin S, Strober S. Spontaneous murine B-cell leukaemia. Nature 272: 624-626, 1978. PubMed: 306069