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74 years adult
肿瘤
HTB-126™
贴壁生长
Designations: | Hs 578T | ||
Depositors: | AJ Hackett | ||
Biosafety Level: | 1 | ||
Shipped: | frozen | ||
Medium & Serum: | See Propagation | ||
Growth Properties: | adherent | ||
Organism: | Homo sapiens | ||
Morphology: | epithelial |
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Source: | Organ: mammary gland; breast Disease: carcinoma |
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Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
Receptors: | estrogen receptor, not expressed [1119] | ||
Tumorigenic: | No | ||
DNA Profile (STR): | Amelogenin: X CSF1PO: 13 D13S317: 11 D16S539: 9,12 D5S818: 11 D7S820: 10 THO1: 9,9.3 TPOX: 8 vWA: 17 |
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Cytogenetic Analysis: | Number of cells examined = 50; Modal Chromosome Number = 59 with a range of 50 to 77; Polyploidy Rate = 33.8% Composite karyotype: 50-77 <3n> X, -1, del(1)(q12), -2, del(2)(?q36), der(3)t(3;15)(q10;p10),-4, -5,der(5)t(5;8)(p10;q10),-6, i(6)(p10), +8, -9, -10, -11, del(11)(p12), -12, -13, -14, -15, -15, -16, -17, -17, -17, i(17)(q10), -18, -19,der(19)(19pter<-q13::5q13<-qter), +22, +3 mar[cp12] |
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Isoenzymes: | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1 Me-2, 0 PGM1, 1 PGM3, 1 |
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Age: | 74 years adult | ||
Gender: | female | ||
Ethnicity: | Caucasian | ||
Comments: | The Hs 578T cell strain was derived from a carcinoma of the breast. It was originated by A.J. Hackett, et al. along with the Hs 578Bst (see ATCC HTB-125), which is a normal fibroblast-like line from the same patient. The Hs 578T line had a mixed polygonal morphology initially, but a stellate cell type was selected for during passage and by cloning. Aggregates of casein protein granules, desmosomes, tight junctions, lipid droplets and vesicularized smooth endoplasmic reticulum were observed by electron microscopy. As with Hs 578Bst, no estrogen receptors or endogenous viruses were detected. Cytogenetics: Derivatives and Markers There are 8 consistent derivative chromosomes: del(1)(q12), del(2)(?q36), der(3)t(3;15)(q10;p10), der(5)t(5;8)(p10;q10), i(6)(p10), del(11)(p12), i(17)(q10), der(19)(19pter<-q13::5q13<-qter) plus two markers of unknown origin and one minute chromosome. Four other markers, including two derivative chromosome 1s were noted are lower frequency. Cytogenetics: Comments This is a hypotriploid human cell line with a modal chromosome number of 59. QM staining verified the absence of a Y chromosome. The rate of polyploidy in excess of the modal number is 33.8%. There were 8 consistent derivative chromosomes: del(1)(q12), del(2)(?q36), der(3)t(3;15)(q10;p10),der(5)t(5;8)(p10;q10), i(6)(p10), del(11)(p12),i(17)(q10), der(19)(19pter<-q13::5q13<-qter) plus two markers of unknown origin and one minute chromosome. Normal chromosome 17's were absent and only a single normal 15 was seen in most cells. No brightly fluorescent Y chromosomes were detected with QM staining. |
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Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 0.01 mg/ml bovine insulin; fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended Medium Renewal: 2 to 3 times per week |
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Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 normal (or near-normal) cell line established from the same patient:ATCC HTB-125 purified DNA:ATCC HTB-126D purified RNA:ATCC HTB-126R |
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References: | 1119: Hackett AJ, et al. Two syngeneic cell lines from human breast tissue: the aneuploid mammary epithelial (Hs 578T) and the diploid myoepithelial (Hs 578Bst) cell lines. J. Natl. Cancer Inst. 58: 1795-1806, 1977. PubMed: 864756 22543: Smith HS. In vitro properties of epithelial cell lines established from human carcinomas and nonmalignant tissue. J. Natl. Cancer Inst. 62: 225-230, 1979. PubMed: 283258 32275: Littlewood-Evans AJ, et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997. PubMed: 9393764 |
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